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Reproducibility of HERMES-measured GABA+ and glutathione in the mesial temporal lobe

2025· article· en· W4412601428 on OpenAlexafffund
Marilena M. DeMayo, Mary Botros, Tiffany Bell, Mark E. Mikkelsen, Victoria Mosher, Antis G. George, Alexander McGirr, Paolo Federico, Ashley D. Harris

Bibliographic record

VenueJournal of Neuroscience Methods · 2025
Typearticle
Languageen
FieldMedicine
TopicAdvanced MRI Techniques and Applications
Canadian institutionsHotchkiss Brain InstituteUniversity of CalgaryOntario Brain InstituteAlberta Children's Hospital
FundersCanadian Institutes of Health ResearchAlberta Children's Hospital Research InstituteNatural Sciences and Engineering Research Council of CanadaHotchkiss Brain Institute
KeywordsReproducibilityNeuroscienceTemporal lobePsychologyGlutathioneBiologyChemistryChromatographyBiochemistryEpilepsy

Abstract

fetched live from OpenAlex

BACKGROUND: There is growing interest in using Hadamard Encoding and Reconstruction for MEGA-Edited Spectroscopy (HERMES) within the mesial temporal lobe (MTL). For cross-sectional group comparisons and longitudinal repeated measures designs, an understanding of the internal and test-retest validity of γ-aminobutyric acid (GABA+) and glutathione (GSH) is critical. We therefore evaluated the reproducibility of the consensus recommended semi-localization by adiabatic selective refocusing (sLASER) localization for edited-MRS acquisitions in a challenging region, the MTL. NEW METHOD: Data were acquired in 15 participants. Single voxel HERMES was collected in the left MTL (two acquisitions) and the right MTL (one acquisition). Participants were repositioned between the two left HERMES acquisitions. An ANOVA was used to assess differences between acquisitions. To assess measurement variation in the repeated left of GABA+ and GSH measures within the left MTL difference values and coefficients of variation (CVs) were calculated. RESULTS: There were no significant differences in metabolite values between any of the acquisitions. The mean difference between the metabolite measures from the repeated left acquisitions centred close to zero, and the average CVs were 14.09 % for GABA+ and 18.94 % for GSH. COMPARISON WITH EXISTING METHODS: The CVs of GABA+ and GSH in the MTL obtained from a HERMES acquisition were comparable to GABA+ or GSH-edited acquisitions in this region, and to data from cortical voxels using HERMES acquisitions. CONCLUSIONS: This supports the use of HERMES in the MTL, a challenging region for MRS. However, larger samples and caution in interpretation may be required in repeated-measures designs.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.007
metaresearch head score (Gemma)0.003
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.804
Threshold uncertainty score0.418

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0070.003
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.087
GPT teacher head0.477
Teacher spread0.390 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations2
Published2025
Admission routes2
Has abstractyes

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