High genomic stability of Andes virus following successive passage <i>in vivo</i> in Syrian hamsters
Bibliographic record
Abstract
ABSTRACT Andes virus (ANDV) is a South American hantavirus that causes hantavirus cardiopulmonary syndrome (HCPS), a severe respiratory infection with a case fatality rate as high as 40%. A critical model for studying HCPS is the Syrian hamster model, which faithfully recapitulates key aspects of the disease. Recent studies have shown that strains of ANDV from different origins, i.e., from rodent or human hosts, can produce widely different outcomes in the hamster model. CHI-7913, a human isolate, does not cause lethal disease and replicates poorly in hamster tissues compared with the classical rodent isolate Chile-9717869. Here, we passaged CHI-7913 in vivo in Syrian hamsters 25 times and infected groups of hamsters with virus from passages 2, 12, and 24 to assess whether viral adaptation occurs that might ultimately drive the phenotype of the virus during infection in this model. Very few mutations occurred during passage in hamsters, with only a single coding mutation discovered after 25 passages. Nearly all mutations occurring in hamsters occurred early, within two passages, and none of the passaged virus caused overt disease or pathology in infected hamsters, consistent with minimal changes in the viral genome. Early in infection, CHI-7913 viral RNA levels were still well below those seen in lethal Chile-9717869 infection. Overall, our data offer insights into the lack of selective pressure on hantaviruses in certain hosts and further evidence of distinct outcomes between rodent and human-derived viral isolates that should be studied further in additional viral species. IMPORTANCE Infection of Syrian hamsters with Andes virus (ANDV) can result in disparate outcomes, depending on the source of the virus used for the infection. The ANDV strain CHI-7913 does not cause lethal disease in hamsters but is able to replicate in hamster tissues. We successively passaged CHI-7913 in vivo to study how continued infection influences adaptation in hamsters and whether passaging would lead to the development of a lethal model, as sometimes occurs with other viruses. Surprisingly, even after 25 passages, minimal mutations occurred in ANDV CHI-7913 genomes, with only one coding mutation present above consensus by passage 25, in the viral glycoprotein. Our data suggest that depending on both viral origin and the host, hantaviruses may face minimal selective pressure to mutate toward a disease phenotype. Studies with additional ANDV strains and other hantavirus species are warranted to further study this phenomenon.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".