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Record W4412838766 · doi:10.1101/2025.07.31.666798

Surveillance on California dairy farms reveals multiple possible sources of H5N1 transmission

2025· preprint· en· W4412838766 on OpenAlexaff
A. J. Campbell, Meredith J. Shephard, Abigail P. Paulos, Matthew D. Pauly, Michelle N. Vu, Chloe Stenkamp-Strahm, Kaitlyn Bushfield, Betsy Hunter-Binns, Orlando Sablon, Emily E. Bendall, William J. Fitzimmons, Kayla Brizuela, Gracie Quirk, Nirmal Kumar, Brian J. McCluskey, Nishit Shetty, Linsey C. Marr, Jenna J. Guthmiller, Kevin Abernathy, Adam S. Lauring, Blaine Melody, Marlene K. Wolfe, Jason E. Lombard, Seema S. Lakdawala

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typepreprint
Languageen
FieldMedicine
TopicViral gastroenteritis research and epidemiology
Canadian institutionsChemistry Industry Association of Canada
FundersNational Institute of Allergy and Infectious DiseasesAnimal and Plant Health Inspection ServiceEmory UniversityCalifornia Dairy Research FoundationU.S. Department of Agriculture
KeywordsMilkingInfluenza A virus subtype H5N1Transmission (telecommunications)Airborne transmissionBiosecurityVeterinary medicineDairy cattleBiologyGeographyAnimal scienceVirusVirologyMedicineCoronavirus disease 2019 (COVID-19)EcologyInfectious disease (medical specialty)Engineering

Abstract

fetched live from OpenAlex

Abstract Transmission routes of highly pathogenic H5N1 between cows or to humans remain unclear due to limited data from affected dairy farms. We performed air, farm wastewater, and milk sampling on 14 H5N1 positive dairy farms across two different California regions. Infectious virus was detected in the air in milking parlors and in wastewater streams, while viral RNA was found in exhaled breath of cows. Sequence analysis of infectious H5N1 virus from air and wastewater samples on one farm revealed viral variants relevant for potential human susceptibility. Longitudinal analysis of milk from the individual quarters of cows revealed a high prevalence of subclinical H5N1 positive cows. Additionally, a heterogeneous distribution of infected quarters that maintained a consistent pattern over time was observed, inconsistent with shared milking equipment serving as the sole transmission mode. The presence of subclinically infected cows was further supported by detection of antibodies in the milk of animals that exhibited no clinical signs during the H5N1 outbreak on one farm. Our data highlight additional sources and potential modes of H5N1 transmission on dairy farms.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.069
Threshold uncertainty score0.138

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0010.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.021
GPT teacher head0.273
Teacher spread0.252 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations11
Published2025
Admission routes1
Has abstractyes

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Same venuebioRxiv (Cold Spring Harbor Laboratory)Same topicViral gastroenteritis research and epidemiologyFrench-language works237,207