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Record W4413031473 · doi:10.1101/2025.08.01.25332809

Advancing Non-Invasive Respiratory Diagnostics: Multiplex Nasal Biomarker Profiling for Stratification of Airway Inflammatory Diseases

2025· preprint· en· W4413031473 on OpenAlexaff
Tanya Lupancu, Sharmala Thuraisingam, Eldin Rostom, David M. Yen, Brian Wang, Adam M. Damry

Bibliographic record

VenuemedRxiv · 2025
Typepreprint
Languageen
FieldMedicine
TopicSinusitis and nasal conditions
Canadian institutionsUniversity of Ottawa
Fundersnot available
KeywordsMultiplexProfiling (computer programming)BiomarkerRespiratory systemMedicineBiomarker discoveryAirwayRisk stratificationImmunologyInternal medicineBioinformaticsBiologyComputer scienceProteomicsSurgeryGene

Abstract

fetched live from OpenAlex

ABSTRACT This study explores the potential of nasal secretions to serve as a source of biomarkers for diagnosing respiratory conditions. A total of 40 inflammatory biomarkers were detected and quantified in participants with upper respiratory diseases, including chronic rhinosinusitis (CRS), allergic rhinitis, and viral and bacterial infections. The different expression levels of various biomarkers could distinguish CRS participants with and without nasal polyps (i.e. CRP, GzmB, IL-4, MMP-1, MMP-8, SAA and TREM-1), and healthy and rhinitis participants (i.e. CRP, EGF, Eotaxin-1, Fractalkine, IL-1RA, IL-5, IP-10 and TRAIL). CRP, G-CSF, GzmA, IL-1, IL-2, IL-5, IL-8, IL-9, MMP-1, TNFa and TREM-1 protein expression differed between the healthy, viral and bacterial-infected individuals, and EGF, G-CSF, MCP-1, MIP1a and MIP-1b protein expression differed between type 2 and non-type 2 inflammatory cohorts. Significant correlations were also noted between SNOT-22 scores and specific cytokines, such as IP-10 and TRAIL. Despite the heterogeneity of patient diagnoses, these findings highlight the potential of nasal fluid as a readily accessible reflection of respiratory health. Future studies with larger cohorts and standardized methodologies are needed to validate these biomarkers and potentially enable precision diagnosis and improved treatment of respiratory conditions.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.003
metaresearch head score (Gemma)0.003
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.016

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0030.003
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0020.001
Open science0.0010.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.028
GPT teacher head0.319
Teacher spread0.291 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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