Guyparkeria halopsychrophila sp. nov., a chemolithoautotrophic polyextremophile isolated from a sub-zero Arctic hypersaline spring
Bibliographic record
Abstract
Two eurypsychrophilic and halophilic sulphur-oxidizing bacterial strains (LHSS19-1 T and GHLCS8-2) were isolated from High Arctic hypersaline sulphur spring sediment samples collected from the Lost Hammer and Gypsum Hill spring systems on Axel Heiberg Island in the Qikiqtaaluk Region, Nunavut, Canada. The strains were obligate chemolithoautotrophs, utilizing reduced sulphur compounds (thiosulphate, sulphide, tetrathionate and elemental sulphur) as electron donors and energy sources. Chemolithoautotrophic growth on thiosulphate was observed at −2 to 37 °C, pH 6.5–9.5 and NaCl concentrations of 1–20 % w/v. The DNA G+C content of the draft genome sequences was 64.59–64.74 mol%. Phylogenetic analyses based on 16S rRNA gene sequences, 92 single-copy core genes and whole genomes indicated that both strains together form a distinct monophyletic clade within the genus Guyparkeria . Moreover, in silico DNA–DNA hybridization, orthologous average nt identity and average aa identity values calculated between each strain and the closest related type strains were ≤69.10%, 87.83% and 90.37%, respectively. The genomes of both strains encoded high O 2 affinity cbb 3 -type cytochrome c oxidase genes but distinctively lacked a complete set of aa 3 -type cytochrome c oxidase genes. The dominant respiratory quinone of strain LHSS19-1 T was ubiquinone-8. The major fatty acids (>10%) were C 18 : 1 ω 7c, C 12 : 0 3OH and C 16 : 0 . Based on phenotypic and genotypic results, strains LHSS19-1 T and GHLCS8-2 represent a novel species within the genus Guyparkeria , for which the name Guyparkeria halopsychrophila sp. nov. is proposed. The type strain is LHSS19-1 T (=NCTC 15100 T =DSM 117890 T ).
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".