A New Strain of <scp>16SrI</scp> Group Phytoplasma Associated With Witches' Broom and Decline of Pacific Willow ( <scp> <i>Salix lucida</i> </scp> )
Bibliographic record
Abstract
ABSTRACT Phytoplasmas are transmitted by phloem‐sucking insect vectors and by vegetative propagation of infected plants. Pacific willow is an ecologically valuable and excellent wetland restoration plant in western North America. Pacific willow trees showing witches' broom (WB) and decline were observed growing on southern Vancouver Island, British Columbia, Canada. Phytoplasma infection was confirmed using a real‐time PCR assay targeting 16S rRNA genes. In silico restriction analysis of the F2nR2 region sequence, with iPhyClassifier, revealed that the restriction pattern of the detected phytoplasma was different from all previously established 16Sr subgroups, and the detected phytoplasma may represent a new strain in the 16SrI group. CpnClassiPhyR analysis of cpn60 universal target (UT) indicated that the detected phytoplasma is a probable new subgroup in Cpn60 UT I. Complete 16S rRNA, rpsP and rplS gene sequences were assembled from short‐read phytoplasma genome sequence data obtained from symptomatic willow trees. Phylogenetic analysis of 16S rRNA, rpsP and rplS genes also confirmed that the phytoplasma detected in WB symptomatic Pacific willow trees represents a new strain belonging to the 16SrI group.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".