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Record W4413128233 · doi:10.1101/2025.08.10.25333051

Comparative analysis of patient-derived organoids and patient-derived xenografts as avatar models for predicting response to anti-cancer therapy

2025· preprint· en· W4413128233 on OpenAlexafffund
Joan Miguel Romero, Jamie Magrill, Nikita Kalashnikov, Michael Luo, Owen J. Chen, Sandrine Busque, Rong Ma, Aline Atallah, Anna-Maria Lazaratos, Daniel Mendelson, Liam Wilson, Shriya Deshmukh, Tarek Taifour, Mark Sorin, Hellen Kuasne, Jeremy Y. Levett, Yifan Wang, Thomas Seufferlein, Alexander Kleger, Johann Gout, Alica K. Beutel, James Brugarolas, Zachry S Poshusta, Tara L. Hogenson, Martín E. Fernández-Zapico, Akinobu Hamada, Shigehiro Yagishita, Anthony C. Nichols, John W. Barrett, Federica Papaccio, Josefa Castillo, Masahiro Inoue, Thierry Massfelder, Hervé Lang, Véronique Lindner, Jonas A. Nilsson, Zahra Dantes, Gabrielle A. Wells, Sung Han Kim, Michael Ittmann, Hugo Villanueva, Seth P. Lerner, Andrew G. Sikora, Charles Theillet, Daniel Q. Huang, Dong-Anh Khuong-Quang, Jonathan Yeung, Peter M. Siegel, Ian R. Watson, George Zogopoulos, April A. N. Rose, Matthew Dankner

Bibliographic record

VenuemedRxiv · 2025
Typepreprint
Languageen
FieldMedicine
TopicCancer Cells and Metastasis
Canadian institutionsUniversity of TorontoWestern UniversityJewish General HospitalUniversity of WindsorMcGill UniversityUniversité de MontréalMcGill University Health Centre
FundersNational Institutes of HealthCanadian Institutes of Health ResearchEuropean Society for Medical OncologyMinistère de l'Économie, de l’Innovation et des Exportations du QuébecMcGill University
KeywordsConcordanceInternal medicineOncologyMedicinePredictive valueCancerOrganoidCancer therapyPsychology

Abstract

fetched live from OpenAlex

Patient-derived xenografts (PDX) and patient-derived organoids (PDO) are widely used to model cancer and predict treatment response in matched patients. However, their predictive accuracy has not been systematically studied nor compared. We conducted a systematic review and meta-analysis of studies using PDX or PDO from solid tumors treated with identical anti-cancer agents as the matched patient, identifying 411 patient-model pairs (267 PDX, 144 PDO). Overall concordance in treatment response between patients and matched models was 70%, with no significant differences between PDX and PDO. Sensitivity, specificity, and positive and negative predictive value were also comparable. Patients whose matched PDO responded to therapy had prolonged progression-free survival. For PDX, this association held only when analyses were restricted to patient-model pairs with low risk of bias after applying a bias assessment metric. Together, these findings suggest that in some contexts, PDO perform similarly to PDX in predicting matched-patient response while potentially offering lower financial and ethical burdens. Given that both platforms have distinct strengths and weaknesses, they continue to serve complementary roles in translational cancer research. Additional prospective studies will be required before definitive recommendations can be made.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.039
metaresearch head score (Gemma)0.073
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.039
Threshold uncertainty score0.207

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0390.073
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0050.014
Bibliometrics0.0030.003
Science and technology studies0.0000.001
Scholarly communication0.0030.001
Open science0.0010.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.045
GPT teacher head0.336
Teacher spread0.291 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2025
Admission routes2
Has abstractyes

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