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Record W4413185941 · doi:10.1016/j.rvsc.2025.105842

Transcriptomic profiling of mink spleen infected by Aleutian mink disease virus: Insight into immune response

2025· article· en· W4413185941 on OpenAlexafffund
DG Bishop, A. Farid, Duy Ngoc, Younes Miar

Bibliographic record

VenueResearch in Veterinary Science · 2025
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicAnimal Virus Infections Studies
Canadian institutionsDalhousie University
FundersNova Scotia Mink Breeders AssociationNatural Sciences and Engineering Research Council of CanadaAtlantic Canada Opportunities AgencyDepartment of Agriculture, Nova ScotiaCanada Mink Breeders Association
KeywordsMinkImmune systemBiologySpleenTranscriptomeVirologyVirusAmerican minkImmunologyGeneGene expressionGenetics

Abstract

fetched live from OpenAlex

Aleutian disease (AD) is one of the most challenging mink diseases that causes high mortality and affects several economically important traits. Aleutian mink disease virus (AMDV) targets multiple organs, the spleen being one of the major targeted organs. Transcriptomics has been widely used to reveal genes and biological pathways and to identify biomarkers for early detection or prevention of diseases. This study aimed to identify genes and pathways related to the host response to AD infection in the spleen using transcriptomics. For this purpose, twelve AMDV-free black male mink (3 sets of 4 full-sibs) were housed for 4–7 days pre-inoculation and then were intranasally inoculated with viral homogenate and euthanized at 24 h (Day 1), 48 h (Day 2), and Day 7 post-infection. Spleen tissues were collected from infected and control (Day 0) mink for RNA isolation. Libraries were prepared (Illumina TruSeq™ RNA kit) and sequenced (HiScanSQ, 101 bp paired-end reads). After cleaning with Cutadapt v1.4.2, 942,803,540 high-quality reads remained for analysis. Differential gene expression analyses revealed that the highest number of significant differentially expressed (DE) genes were observed between Day 1 and Day 0 (168) while the fewest DE genes (23) were observed between Day 7 and Day 0. A total of 19 DE genes are identified between three observation pair comparisons and eight of them are directly involved in immune response ( FGL2, TLF8, LRP1, SERPINB9, MSR1, C3, PLA2R1 , and XCR1) . Gene enrichment analyses revealed pathways related to innate immune and lipid metabolism which are important mechanisms for the host to fight against infections. One of the important pathways is neutrophil degranulation which functions as aiding in the elimination of pathogens and the initiation of the inflammatory process. In conclusion, the current study provides insight into the transcriptomic profiles of the spleen in mink infected with AMDV. Identified candidate genes might be used for functional studies or as prior information for markers or genomic selection against AD in mink. Further studies in other tissues or single-cell RNA sequencing might deliver more comprehensive pictures of host responses to AMDV infections. • Spleen transcriptomic profiles of mink infected with Aleutian disease virus were performed on Day 0, Day 1, Day 2 and Day 7. • Profiles from Day 1, Day 2 and Day 7 were compared to Day 0 (control). • 19 differentially expressed genes are identified between the three observation pair comparisons (Day 0 vs infection Day 1, 2, or 7)). • Eight common dysregulated genes ( FGL2, TLF8, LRP1, SERPINB9, MSR1, C3, PLA2R1 , and XCR1) are involved in immune function . • Innate immune and lipid metabolism were significantly enriched pathways. • The most interactive genes in network analyses included CD86, MMP9, VCAM, PPARG, PDGFRA, SMG1, SLCA7, GATA1, CCL5 and C3.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.003
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.882
Threshold uncertainty score0.718

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0030.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.006
Science and technology studies0.0010.002
Scholarly communication0.0000.000
Open science0.0010.001
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.082
GPT teacher head0.373
Teacher spread0.290 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

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Citations0
Published2025
Admission routes2
Has abstractyes

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