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Record W4413363082 · doi:10.3389/fvets.2025.1649751

Associations between prepartum urine pH and periparturient blood calcium concentrations in multiparous Holstein cows

2025· article· en· W4413363082 on OpenAlexaff
Jesse P. Goff, Pedro Meléndez, J.A. Bartolomé, T.R. Overton, B.M. Leno, J.K. Drackley, K.M. Glosson, Xiangfei Zhang, S.J. LeBlanc, Rita Couto-Serrenho, J.E.P. Santos, C. Lopera, R. Zimpel, R.M. Rodney, I.J. Lean

Bibliographic record

VenueFrontiers in Veterinary Science · 2025
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicReproductive Physiology in Livestock
Canadian institutionsUniversity of Guelph
Fundersnot available
KeywordsUrineChemistryNadirEndocrinologyAnimal scienceCalciumInternal medicineBiologyMedicineBiochemistry

Abstract

fetched live from OpenAlex

Introduction: Metabolic alkalosis induced by prepartum diet cations impairs Ca homeostasis in the periparturient cow. Adding anions to prepartum diets reduces blood pH improving periparturient Ca homeostasis. Urine pH generally reflects blood pH and is practical to measure on farm. The degree to which urine should be acidified to increase periparturient blood Ca concentration is not well defined. Materials and methods: Prepartum urine pH and periparturient blood Ca concentrations determined in 660 multiparous Holstein cows from 9 studies were analyzed. Least Square Means of the lowest blood Ca concentration (Ca nadir) observed in cows within 6 urine pH categories (≤ 5.75, 5.76 to 6.25, 6.26 to 6.75, 6.76 to 7.25, 7.26 to 7.75, and ≥ 7.76) were determined. Data were analyzed across all 660 cows and then by parity. Results: Across all cows, the Ca nadir was lowest and the incidence of cows with Ca nadir < 2.00 mM, indicative of subclinical hypocalcemia (SCH), was greatest when urine pH was ≥ 7.76. Mean Ca nadir increased in all cows with urine pH < 7.75. There was no significant difference in Ca nadir or % of cows with SCH when prepartum urine pH was < 7.75. This was also the case for 2nd and 3rd parity cows. However, in ≥ 4th parity cows, those with urine pH between 6.26 and 6.75 had significantly higher Ca nadir than cows with urine pH above 7.25 or below 5.75. Calcium nadir and blood Ca concentrations at 2 days in milk (DIM) were highly correlated (r = + 0.58), and blood Ca concentrations at 2 and 4 DIM were moderately correlated (r = + 0.43). Blood Ca concentration at 4 DIM was weakly associated with Ca nadir (r = + 0.30). Discussion: These analyses support acidification of cows to achieve prepartum urine pH below 7.75 to increase blood Ca nadir for 2nd and 3rd parity cows. For ≥ 4th parity cows, the highest blood Ca nadir was observed with urine pH below 7.25 and above 5.75. For ≥ 4th parity cows, urine pH below 5.75 was associated with significantly lower blood Ca nadir.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.028
GPT teacher head0.278
Teacher spread0.250 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2025
Admission routes1
Has abstractyes

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