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Record W4413777741 · doi:10.1101/2025.08.27.672573

Methanotroph Dynamics at Landfill Cover Soil Methane Emission Hotspots

2025· preprint· en· W4413777741 on OpenAlexaffabout
Emmanuelle Roy, Henry Gibbons, Maria Strack, Laura A. Hug

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typepreprint
Languageen
FieldEnvironmental Science
TopicLandfill Environmental Impact Studies
Canadian institutionsUniversity of Waterloo
Fundersnot available
KeywordsMethanotrophMethaneCover (algebra)Land coverEnvironmental scienceMethane emissionsAnaerobic oxidation of methaneLand useEcologyEngineeringBiology

Abstract

fetched live from OpenAlex

Abstract Landfills contribute significant emissions to the global methane cycle, where emission hotspots can account for the majority of methane released. Methanotrophs in landfill cover soils can mitigate these methane emissions, but are constrained by geochemical conditions in the soils and the climate at the landfill’s location. We sampled cover soils from four Ontario landfills with differing characteristics, including measurements of methane flux, soil methane concentration, and a suite of geochemical variables. Sampling sites were distinguished based on the levels of methane flux. Microbial community diversity and methanotroph dynamics were examined using 16S rRNA gene amplicon sequencing. Sites with high methane emissions showed strong enrichment of methanotrophs, dominated by the genus Methylomicrobium . The distribution of methanotrophs in samples across landfills and sites differed significantly when considering community evenness. Of the environmental factors examined, microbial community diversity correlated most strongly to nitrate and nitrite concentrations. Methanotroph dynamics across different methane exposures and geochemical conditions within landfill cover soils inform the use of designer cover soils and/or methanotroph amendments in efforts toward mitigating methane emissions from landfills.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.107
Threshold uncertainty score0.212

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0010.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.217
Teacher spread0.207 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes2
Has abstractyes

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