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Record W4414048459 · doi:10.1101/2025.08.31.673387

Towards Fully Automated Investigation of Social Learning in Mice

2025· preprint· en· W4414048459 on OpenAlexaff
Benjamin Lang, Christa Thöne‐Reineke, Olaf Hellwich, Lars Lewejohann

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicZebrafish Biomedical Research Applications
Canadian institutionsScience North
Fundersnot available
KeywordsSocial learningIdentification (biology)Prosocial behaviorSocial animalAssociation (psychology)Social behaviorTracking (education)Social relation

Abstract

fetched live from OpenAlex

Abstract Mice have been demonstrated to learn from each other in social interactions, the extent to which this takes place and the strategies involved, however, largely remain to be elucidated beyond spatially and temporally confined tests of social memory retention. Here, we present a method which utilizes and modifies 1) a commercially available tool for automated behavioral testing, the IntelliCage and 2) an open-source solution for 24/7 live animal tracking, the Live Mouse Tracker, to create a powerful method for the investigation of learning behavior in semi-naturalistic group settings. We see a wide range of possible applications, such as for instance the investigation of learning in social interactions, which we present here as an example. In the present study, co-learning did not facilitate place learning over individual learning. While automated annotation of behaviors was effective, markerless animal identification proved unreliable in a highly enriched environment with manifold opportunity of occlusion from video tracking. In response, we here present a rationale for identifying the reliable portion of tracking data, to which we confine the behavioral analysis. Co-learning animals engaged more often in some prosocial interactions with their teammates than with other animals, but they did overall not interact with each other more frequently than with individual learners. Correlative analysis of learning behavior and social interactions did not reveal any particular association between behavior and learning success. While the mechanisms of social learning in mice could not be conclusively elucidated within the scope of this study, we report on the development of a promising tool for presenting manifold learning tasks to mice while tracking their individual and social behaviors in a fully automated manner. Further, we discuss limitations of the current configuration and present an outlook on further improving the method.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.001
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0010.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.268
Teacher spread0.256 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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Same venuebioRxiv (Cold Spring Harbor Laboratory)Same topicZebrafish Biomedical Research ApplicationsFrench-language works237,207