An Aurora Kinase A/TPX2 complex phosphorylates CKAP2 to control mitotic spindle growth
Bibliographic record
Abstract
Summary Faithful chromosome segregation requires the precise assembly of the mitotic spindle. Cytoskeleton-associated protein 2 (CKAP2) is a microtubule-associated protein with potent microtubule-polymerizing activity that localizes to spindle microtubules during mitosis. Loss or overexpression of CKAP2 causes chromosomal instability and aneuploidy, yet its regulation remains poorly understood. To identify CKAP2 interactors, we immunoprecipitated endogenous CKAP2 from mitotic RPE1 cells and analysed co-purifying proteins by mass spectrometry. This revealed a specific interaction between CKAP2 and the mitotic kinase Aurora A and its activator TPX2, but not with Aurora B, as previously suggested. We further show that CKAP2 co-localizes with Aurora A-TPX2 complexes throughout mitosis, and Aurora A directly phosphorylates CKAP2 in cells and in vitro. Phosphorylation of CKAP2 By Aurora kinase A decreases its microtubule affinity in cells and in vitro. These findings identify CKAP2 as a direct interactor of TPX2 and a substrate of Aurora kinase A and uncover a regulatory pathway controlling spindle growth and stability during mitosis.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.004 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".