Molecular detection of <i>Rickettsia felis</i> in <i>Dermacentor albipictus</i> from Mississippi
Bibliographic record
Abstract
Dermacentor albipictus (Packard), the winter tick, is a one-host tick often found in high numbers on horses, moose, elk, and deer in the northern United States and parts of Canada. In Mississippi, there have been very few collections of D. albipictus. Herein we report the collection and molecular screening of 5 male specimens and 3 nymphs of the winter tick for rickettsial organisms. Broad PCR screening using a PanRickettsia TaqMan PCR assay targeting a portion of the 23 s gene was utilized, and a family-wide Anaplasmataceae SYBR real-time PCR assay was chosen for initial screening of tick eluates. PanRickettsia PCR-positive samples were then amplified using a conventional PCR targeting the citrate synthase (gltA) gene for species identification. One resultant amplicon was 99.75% identical to Rickettsia felis DNA. This is the first report of R. felis in the winter tick, D. albipictus.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".