Nuclear lamina-associated domain biogenesis is regulated by nuclear pore density during embryogenesis and mediates UV protection
Bibliographic record
Abstract
SUMMARY Lamina-associated domains (LADs) are critical for genome organization and function, but their formation during development is not well understood. Here, we use DamID and image analysis to reveal the dynamics of LAD biogenesis during C. elegans embryogenesis. At early stages, DNA at the lamina is transcriptionally active and lacks lamina-associated heterochromatin. This state depends on abundant nuclear pores, which prevent heterochromatin accumulation at the nuclear periphery. As development proceeds, pore numbers decline, enabling heterochromatin to access the lamina. Reducing nuclear-pore components induces precocious accumulation of heterochromatin to the lamina. Functionally, we find that heterochromatic LADs confer protection against ultraviolet (UV) radiation. Older embryos are resistant to UV light and concentrate DNA damage at the nuclear periphery, whereas early embryos are UV sensitive and accumulate damage throughout nuclei when unshielded by their mothers in utero . These findings identify embryonic dissipation of nuclear pores as a key step in heterochromatic LAD assembly, allowing older embryos to withstand exposure to UV irradiation.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".