Guanine storage vacuoles sequester guanine crystals and serve as nitrogen reserves in <i>Chlamydomonas reinhardtii</i>
Bibliographic record
Abstract
Nitrogen cycles in Chlamydomonas reinhardtii have historically been studied in the context of exogenous ammonium/nitrate availability and uptake. Recently, crystalline guanine has been identified, using Raman microscopy, in the cytoplasm of numerous microalgae, including C. reinhardtii . The signal level is influenced by availability of exogenous nitrogen, suggesting that the deposits serve as a nitrogen store. We report the visualization of these guanine crystals in C. reinhardtii and other microalgae using quick-freeze deep-etch electron microscopy. They prove to reside within cytoplasmic vacuoles: A membrane with a high density of small intramembranous particles surrounds a uniform, coarsely granular interior matrix within which stacks of flat crystals assemble, often in stunning abundance. We designate these as guanine storage vacuoles (GSVs). In C. reinhardtii and other members of the Chlamydomonadales, a discrete disc-shaped granular domain occupies the center of each GSV, and the crystals arise within or at the edges of this domain, suggesting that it represents an amorphous form of guanine. In C. reinhardtii , GSVs are numerous in log-phase cells, are not found in cells maintained in nitrogen-free medium, and reappear within 8 h after the cells are provided with nitrogen, initially as small disc-containing vacuoles. We compare this cycle with that of the polyphosphate granules localized in acidocalcisomes. Nineteen enzyme families and 10 transporters are predicted to participate in guanine-related pathways in C. reinhardtii , many showing strong upregulation with nitrogen starvation. These understandings will inform future studies on the contributions made by GSVs to the metabolism and physiology of eukaryotic microalga.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".