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Record W4415371423 · doi:10.1016/j.ejmcr.2025.100302

The impact of secondary site interactions on the binding specificity of bitopic dopamine D2/D3 receptor ligands

2025· article· en· W4415371423 on OpenAlexafffund
Attila Egyed, Dóra Kiss, Zsófia Pável, Keve Rajna, Supriya A. Gaitonde, Mariam Dubiel, Marı́a Isabel Loza, Edina Szűcs, Éva Borbély, Zsófia Hajna, Grzegorz Satała, Márton Vass, Pál Szabó, Katalin Jósvay, Andrzej J. Bojarski, Holger Stark, Zsuzsanna Helyes, Erika Pintér, Michel Bouvier, György M. Keserű

Bibliographic record

VenueEuropean Journal of Medicinal Chemistry Reports · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicReceptor Mechanisms and Signaling
Canadian institutionsUniversité de MontréalInstitute for Research in Immunology and Cancer
FundersNemzeti Kutatási, Fejlesztési és Innovaciós AlapÁltalános Orvostudományi Kar, Pécsi TudományegyetemNemzeti Kutatási Fejlesztési és Innovációs HivatalNemzeti Kutatási és Technológiai HivatalMagyar Tudományos AkadémiaHungarian Scientific Research FundDeutsche ForschungsgemeinschaftCanadian Institutes of Health ResearchHungarian Research Network
KeywordsG protein-coupled receptorReceptorDopamine receptor D2Dopamine receptorDopaminergicDopamine receptor D3DopamineFunctional selectivityBinding site

Abstract

fetched live from OpenAlex

Dysfunction of dopamine receptors and interruption of normal dopaminergic signaling have been linked to severe neuropsychiatric diseases such as schizophrenia, Parkinson's disease and depression. Although these receptors are among the most drug-targeted families of GPCRs, designing compounds with subtype-specific pharmacological profile remains challenging due to the structural similarities among the subtypes of the family. Considering the structural moieties of cariprazine, a prototypic drug with D 3 receptor (D 3 R) preference, we report a library of bitopic analogues to explore how the orthosteric and secondary binding motifs, coupled with different linkers, affect D 2 R/D 3 R selectivity and activity. Compounds with promising dopamine receptor selectivities and binding affinities were further characterized on a GPCR panel and in functional assays. Our efforts resulted in an advanced lead compound ( 20b ) with improved D 3 R and 5-HT 2A R, as well as decreased D 2 R and 5-HT 1A R potencies (EC 50 ) compared to cariprazine. The cleaner receptor profile of this compound retained activities for the most important antidepressant mechanisms that were confirmed by reducing the depression-like behavior in mice.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.009
Threshold uncertainty score0.309

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0020.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.263
Teacher spread0.253 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes2
Has abstractyes

Explore more

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