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Record W4415387709 · doi:10.1038/s41598-026-53398-3

LYL1 is critical for adult hematopoietic stem cell-derived B and T lymphopoiesis

2025· preprint· en· W4415387709 on OpenAlexafffund
Sung Kai Chiu, James The, Jesslyn Saw, Jacqueline Boyle, David J. Curtis, Cédric S. Tremblay

Bibliographic record

VenueScientific Reports · 2025
Typepreprint
Languageen
FieldMedicine
TopicAcute Myeloid Leukemia Research
Canadian institutionsUniversity of Manitoba
FundersMedical Research CouncilUniversity of ManitobaNational Health and Medical Research CouncilCancerCare Manitoba Foundation
KeywordsLymphopoiesisHaematopoiesisProgenitor cellConditional gene knockoutTranscription factorStem cellProgenitorLineage (genetic)Transplantation

Abstract

fetched live from OpenAlex

Hematopoietic stem cells (HSCs) are defined by their remarkable self-renewal and differentiation capacities, giving rise to progenitors that generate diverse blood and immune cell lineages through the tightly regulated process of hematopoiesis. This process is governed by key transcription factors that control gene expression programs essential for cellular homeostasis, differentiation, and lineage commitment. The transcription factor Lyl1 plays a critical role in regulating HSC function and the lineage specification of hematopoietic progenitors. To investigate its role in adult hematopoiesis, we developed a novel conditional Lyl1 knockout (KO) mouse model. Conditional deletion of Lyl1 in adult mice resulted in reduced HSC and lymphoid progenitor populations, consistent with findings from constitutive Lyl1 KO models. Despite these reductions, mature B- and T-lymphocyte numbers remained unchanged, in contrast to previous reports from embryonically deleted Lyl1 models. Competitive transplantation assays further confirmed that Lyl1 is essential for the generation of adult HSC-derived lymphoid progenitors that support adaptive T- and B-cell production. Our new conditional Lyl1 KO model provides a valuable tool for dissecting Lyl1 function across developmental stages and uncovering the mechanisms that maintain postnatal hematopoietic homeostasis.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.008

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.001
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0020.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.024
GPT teacher head0.323
Teacher spread0.298 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes2
Has abstractyes

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