Metabolite profiling, cytotoxicity, liver ROS detoxifiers and acetylcholinesterase inhibitors from Trachyandra ciliata L.F. (Kunth) (wild cabbage)
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Bibliographic record
Abstract
• For the first time, Metabolite profiling, cytotoxicity, liver ROS detoxifiers and acetylcholinesterase inhibitors from Trachyandra ciliata were evaluated in quest to discover more plant-based nutraceuticals for the treatment of chronic diseases. • A total of 71 compounds were observed. • Root extract obtained from 0 mM salinity treatment at 0.5 mg/mL extract concentration showed moderate toxicity to H4IIE- luc cells, while it was non-cytotoxic (> 80 %) to non-cancer cells. • Ethanolic extracts of flower buds from plants grown under 100 mM NaCl and roots from plants grown under 100 and 150 mM NaCl had higher anti-AChE activity. • These findings suggest that T. ciliata could potentially be a therapeutic agent for the treatment of cancer, Alzheimer's disease, and liver disorders. Wild cabbage ( Trachyandra ciliata ) is one of the understudied, wild, edible halophytes from South Africa. Although its edibility has recently been validated, its therapeutic potential was yet to be explored. This research was carried out to profile and characterise the phytochemical content of T. ciliata extracts and evaluate its potential as a therapeutic agent for cancer, acetylcholinesterase (AChE) inhibition and reactive oxygen species (ROS) scavenging in the liver. Cuttings of T. ciliata were grown under 0, 50, 100, 150, and 200 mM salinity concentrations. The ultra-high-performance liquid chromatography mass spectrometry (UHPLC-MS) was employed to quantify and characterise metabolites in leaves, roots, and flower buds of T. ciliata . The yellow dye 3-(4,5-dimethyltiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT), Ellman's colourimetric method, and the 2′,7′-dichlorodihydrofluorescein diacetate assay (H 2 DCF-DA) were respectively employed to evaluate cytotoxicity, AChE antagonism, and ROS scavenging of the extracts of T. ciliata . A total of 71 compounds were observed, which were grouped into flavonoids, anthocyanins, alkaloids, nucleobase, nucleosides/tide, saccharides, fatty acids, amino acids, and coumarins. A concentration of 1 mg/mL of extracts of T. ciliata flower buds prepared from plants grown at 0 mM and 100 mM salinity, showed strong cytotoxicity to cancer cells, however, the extracts also had moderate and weak cytotoxicity to non-cancer cells. All extracts inhibited AChE activity. Moreover, ROS scavenging was mainly observed primarily in the extracts of leaves from plants grown at all salinities, and in the extract of roots from plants grown at 0 mM salinity treatment. A maiden documentation of anticancer, acetylcholinesterase inhibition, and ROS scavenging activity of crude extracts of T. ciliata was achieved in this study. These findings suggest that T. ciliata could be a potential therapeutic agent for the treatment of cancer, Alzheimer's disease, and liver disorders, amidst the quest to develop more plant-based pharmaceuticals for the treatment of chronic diseases.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.002 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it