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Record W4415603055 · doi:10.1083/jcb.202409169

Moesin controls cell–cell fusion and osteoclast function

2025· article· en· W4415603055 on OpenAlexafffund
Ophélie Dufrançais, Marianna Plozza, Marie Juzans, Arnaud Métais, Sarah Monard, Pierre‐Jean Bordignon, Perrine Verdys, Thibaut Sanchez, Martin Bergert, Julia Halper, Christopher J. Panebianco, Rémi Mascarau, Rémi Gence, Gaëlle Arnaud, Myriam Ben Neji, Isabelle Maridonneau‐Parini, Véronique Le Cabec, Joel D. Boerckel, Nathan J. Pavlos, Alba Diz-Muñoz, Frédéric Lagarrigue, Claudine Blin‐Wakkach, Sébastien Carreno, Renaud Poincloux, Janis K. Burkhardt, Brigitte Raynaud-Messina, Christel Vérollet

Bibliographic record

VenueThe Journal of Cell Biology · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicBone Metabolism and Diseases
Canadian institutionsUniversité de Montréal
FundersNational Institute of Arthritis and Musculoskeletal and Skin DiseasesNational Institute of Allergy and Infectious DiseasesCanadian Institutes of Health ResearchNational Institutes of HealthAgence Nationale de la RechercheWellcome TrustUniversité Toulouse III - Paul SabatierNational Institute of General Medical SciencesLigue Contre le CancerFondation Toulouse Cancer SantéCentre National de la Recherche ScientifiqueEuropean Molecular Biology LaboratoryInstitut National de la Santé et de la Recherche MédicaleEMBL AustraliaFondation pour la Recherche MédicaleCancer Research Foundation
KeywordsOsteoclastMoesinCytoskeletonCell fusionMultinucleateSyncytiumBone resorptionLipid bilayer fusionActin cytoskeleton

Abstract

fetched live from OpenAlex

Cell-cell fusion is an evolutionarily conserved process that is essential for many functions, including the formation of bone-resorbing multinucleated osteoclasts. Osteoclast multinucleation involves dynamic interactions between the actin cytoskeleton and the plasma membrane that are still poorly characterized. We found that moesin, a cytoskeletal linker protein member of the Ezrin, radixin, and moesin (ERM) protein family, plays a critical role in both osteoclast fusion and function. Moesin inhibition favors osteoclast multinucleation as well as HIV-1- and inflammation-induced cell fusion. Accordingly, moesin depletion decreases membrane-to-cortex attachment and enhances the formation of tunneling nanotubes, F-actin-based intercellular bridges triggering cell-cell fusion. In addition, moesin regulates the formation of the sealing zone, a key structure determining osteoclast bone resorption area, and thus controls bone degradation via a β3-integrin/RhoA/SLK pathway. Finally, moesin-deficient mice have reduced bone density and increased osteoclast abundance and activity. These findings provide a better understanding of cell-cell fusion and osteoclast biology, opening new opportunities to specifically target osteoclasts in bone disease therapy.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.003
GPT teacher head0.209
Teacher spread0.206 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations8
Published2025
Admission routes2
Has abstractyes

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