A Non‐G‐Quadruplex Hemin Aptamer Forms a Better Peroxidase Mimicking DNAzyme
Bibliographic record
Abstract
G‐quadruplex DNA is known to bind to hemin, forming a complex that exhibits peroxidase‐like activity. A non‐G‐quadruplex aptamer named Hem1‐2T also exhibits horseradish peroxidase (HRP) like activity upon binding to hemin. Herein, the catalytic characteristics of the Hem1‐2T aptamer are studied and compared with PS2.M, an extensively studied G‐quadruplex. From pH 6–8, the activity of Hem1‐2T decreases with the increase in pH, which is similar to HRP, whereas the activity of PS2.M increases with pH, suggesting that Hem1‐2T might be a better mechanistic mimic of HRP. Additionally, Hem1‐2T is more effective at protecting hemin from degradation by H 2 O 2 , as evidenced by a slower decrease in the absorbance at 404 nm compared to PS2.M and more sustained catalysis. NMR spectroscopy indicates that hemin promotes ligand‐induced structure formation in the Hem1‐2T aptamer and forms a specific complex, whereas hemin interacts with the PS2.M G‐quadruplex in a way leading to the disappearance of NMR peaks. Overall, the Hem1‐2T‐hemin complex is a better and more stable HRP mimic, supporting its potential applications in bioanalysis and biocatalysis.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".