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Record W4415713368 · doi:10.1101/2025.10.29.685130

A hyperglycosylated form of Kv <sub>1.2</sub> upregulated in LGI1 knockout mice

2025· preprint· W4415713368 on OpenAlexaff
Jorge Ramírez‐Franco, Marion Sangiardi, Kévin Debreux, Maya Belghazi, Christian Lévêque, Michael Seagar, Oussama El Far

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typepreprint
Language
FieldMedicine
TopicAutoimmune Neurological Disorders and Treatments
Canadian institutionsCanadian Nautical Research Society
FundersInstitut National de la Santé et de la Recherche MédicaleAgence Nationale de la Recherche
KeywordsDownregulation and upregulationProtein subunitHippocampal formationPhosphorylationKnockout mouseHEK 293 cells

Abstract

fetched live from OpenAlex

Abstract Kv 1 voltage-gated potassium channels determine key functional neuronal properties. Their activity is modulated by subunit composition and post-translational modifications such as phosphorylation and glycosylation. Using an antibody directed against a phosphotyrosine (Y 458 ) located in the C-terminal tail of Kv 1.2 , we identified yet unreported high molecular weight forms of Kv 1.2 among them, a phosphorylated and heavily glycosylated 100 kDa form. Owing to the significant downregulation of Kv 1.2 in LGI1-dependent autosomal dominant lateral temporal lobe epilepsy, we investigated, in total brain and the hippocampal formation of both WT and Lgi1 -/- mice, the distribution of phosphoY 458 Kv 1.2 and we compared their respective proteomic interactomes with those of Kv 1.2 . In addition to major differences between the interactomes of pY 458 Kv 1.2 and Kv 1.2 in WT and Lgi1 -/- , we found a major reshaping of pY 458 Kv 1.2 molecular neighbourhood between WT and Lgi1 -/- as well as a significant upregulation of the glycosylated form in Lgi1 -/- .

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.004
Threshold uncertainty score0.012

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0040.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.217
Teacher spread0.207 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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