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Record W4415741038 · doi:10.1590/0103-8478cr20240596

Isolation of Clostridioides difficile from the feces of equids and ruminants in a teaching veterinary hospital

2025· article· pt· W4415741038 on OpenAlex
Fabrício Moreira Cerri, Roberta Martíns Basso, Lia Mara Netto Dornelles, Thaís Teresa Brandão Cavalheiro Ribeiro, Geraldo Alves Neto, Rodrigo Otávio Silveira Silva, Amanda Haisi, João Pessoa Araújo, José P. Oliveira‐Filho, Luis G. Arroyo, Alexandre Secorun Borges

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueCiência Rural · 2025
Typearticle
Languagept
FieldMedicine
TopicClostridium difficile and Clostridium perfringens research
Canadian institutionsUniversity of Guelph
FundersCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorFundação de Amparo à Pesquisa do Estado de São Paulo
KeywordsFecesClostridioidesIsolation (microbiology)Multiplex polymerase chain reactionAntimicrobialTeaching hospitalPolymerase chain reactionAntibiotic resistance

Abstract

fetched live from OpenAlex

ABSTRACT: Clostridioides difficile is an important enteropathogen in humans and multiple animal species. This study determined the fecal occurrence of C. difficile in hospitalized or healthy equids and ruminants at a veterinary teaching hospital. A total of 84 fecal samples from ruminants and equids and ten environmental swabs were collected. The samples were cultured under anaerobic conditions to isolate C. difficile. DNA was extracted from C. difficile colonies and subjected to multiplex PCR to identify toxin-coding genes. The isolates were ribotyped, with the toxigenic isolates and one selected non-toxigenic isolate submitted for whole-genome sequencing. C. difficile was isolated from 10/84 (12%) of the fecal samples, of which one bovine isolate was toxigenic (A+B+ CDT -), classified as RT 046/ST35/clade 1, and carried five antimicrobial resistance genes (ermB, tetA, tetB, tetM, and inuP). Although, the occurrence of toxigenic strains was low, the study identified isolates kept in the hospital environment, highlighting the need for preventive measures, especially due to the presence of numerous animals undergoing antimicrobial therapy.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.395
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.019
GPT teacher head0.309
Teacher spread0.291 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it