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Record W4415889716 · doi:10.1101/2025.11.02.686142

The genome of <i>Istocheta aldrichi</i> (Diptera: Tachinidae), a parasitoid of the Japanese beetle, <i>Popillia japonica</i> (Coleoptera: Scarabaeidae)

2025· preprint· en· W4415889716 on OpenAlexaboutno aff
Pablo A Stilwell, Jack A Culotta, W. D. Hutchison, Amelia R. I. Lindsey

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2025
Typepreprint
Languageen
FieldAgricultural and Biological Sciences
TopicInsect-Plant Interactions and Control
Canadian institutionsnot available
FundersMinnesota Department of Agriculture
KeywordsGenomeParasitoidGenomicsGenome sizeRange (aeronautics)PEST analysisHost (biology)Gene

Abstract

fetched live from OpenAlex

ABSTRACT Istocheta aldrichi Mesnil 1953 (Diptera: Tachinidae), is native to Japan, and has recently become an important biological control agent of the Japanese beetle, Popillia japonica (Coleoptera: Scarabaeidae), a pest with >300 host plants, including roses, linden trees, and numerous agricultural crops. During the past decade, I. aldrichi ’s range has greatly expanded across North America, particularly in Quebec and Ontario, Canada, and in the Midwest U.S. In many areas, including Minnesota, 15-60% of Japanese beetles are parasitized by I. aldrichi , highlighting its importance as a natural enemy. To facilitate research on I. aldrichi and other tachinid flies we present a reference genome generated from a single individual. The final genome assembly is 875.3 Mbp contained in 1,041 scaffolds, with an N50 of 4.77 Mbp, and 99.5% complete Diptera BUSCOs present. We also present a complete mitogenome and use comparative genomics across 19 tachinid species to identify unique features of I. aldrichi . Specifically, we find that while many tachinid lineages have experienced contractions in gene families, I. aldrichi is characterized by a relatively high number of gene family expansions, many of which are predicted to function in metal ion transport. Tachinids as a whole have undergone rapid copy number changes in 935 gene families, largely related to metabolism and morphogenesis. The I. aldrichi reference genome will further research opportunities on these parasitic flies, including their potential for biocontrol of P. japonica . ARTICLE SUMMARY The parasitic fly Istocheta aldrichi attacks and kills the Japanese beetle ( Popillia japonica ), a pest of more than 300 plants. There is potential to leverage I. aldrichi for biological control of the beetle, but application is hindered by a limited understanding of this fly’s biology. This reference genome for I. aldrichi will enhance research future efforts and our ability to manage P. japonica .

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.028
Threshold uncertainty score0.056

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0010.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.198
Teacher spread0.190 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2025
Admission routes1
Has abstractyes

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