Basophils activate oncostatin M receptor–expressing vagal sensory neurons
Bibliographic record
Abstract
BACKGROUND: Vagal sensory neurons (VSNs) relay signals from internal organs to the brainstem via the vagus nerve. Published transcriptomic data sets suggest that VSNs express Mas-related G protein-coupled receptor family member D and oncostatin M (OSM) receptor, markers also found in dorsal root ganglia-resident pruriceptor neurons. However, the cellular source of OSM and its potential interactions with VSNs remain unknown. OBJECTIVE: We sought to validate the expression of pruritogen receptors on VSNs, identify basophils as a source of OSM, and determine how OSM modulates VSN activity. METHODS: Pruritogen receptor expression on VSNs was validated by immunofluorescence staining, and VSN responses to pruritogens were assessed using in vitro and in vivo calcium imaging. Mouse models of allergic airway inflammation, fluorescence-activated cell sorting, quantitative PCR, and ELISA were used to evaluate OSM expression and production by lung basophils. RESULTS: Immunofluorescence and quantitative PCR confirmed that airway-innervating VSNs express OSM receptor and that lung basophils are enriched for Osm transcripts. Lung-resident basophils produced OSM following FcεRIα engagement, with production further enhanced after sensitization to house dust mite (Dermatophagoides pteronyssinus) or the fungal allergen Alternaria alternata. In vitro and in vivo calcium imaging revealed that OSM sensitizes multiple populations of VSNs. CONCLUSIONS: These findings identify a novel mechanism of communication between basophils and VSNs during type I hypersensitivity reactions, such as those occurring in allergic asthma.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".