Gas Chromatography and Mass Spectroscopy (GC-MS) Analysis of Hibiscus Sabdariffa L. Calyx Extract and It’s Antimicrobial Activity
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Bibliographic record
Abstract
During current investigation, GC-MS analysis was performed to identify various phytochemicals present in various Hibiscus sabdariffa L. calyx extracts e.g. hydroethanolic, ethanolic and ethyl acetate extracts. In hydroethanolic extract 33 phytochemicals were identified from which 2-Hexenedioic acid, 2,4-dichloro-5-oxo- (28.19%), Ethyne, fluoro- (19.11%), Nitrous oxide (10.71%), 5-Hydroxymethylfurfural (8.41%), 1H-Pyrazole-3-carboxylic acid, 2,5-dihydro-5-oxo- (4.67%), Carbon dioxide (4.62%), 3-Pentenoic acid, 3-ethyl-, methyl ester (4.29%), (2-Aziridinylethyl)amine (3.68%) and Ethane, 1-chloro-1-fluoro-(2.75%) were major compounds. In ethanolic extract 53 compounds were found, from which Acetic acid (39.93%), Hexanedioic acid (14.63%), 3-Pentenoic acid, 3-ethyl-, methyl ester (6.34%), Ethyne, fluoro- (5.99%), l-Alanine ethylamide were major compounds,, whereas in ethyl acetate extract total 21 compounds were identified from which Tetracontane (20.32%), n-Hexadecanoic acid (16.32%), Lup-20(29)-en-3-ol, acetate, (3.beta.)- (11.28%), 9,12,15-Octadecatrienoic acid, (Z,Z,Z)- (6.09%) and 9,12-Octadecadienoic acid (Z,Z)-(5.60%) were major compounds. All of the extracts showed antibacterial activities against Escherichia coli, Staphylococcus aureus.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.003 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.002 | 0.002 |
| Science and technology studies | 0.001 | 0.002 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it