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Record W4416374040 · doi:10.14785/lymphosign-2025-0008

A novel missense variant in CTLA4 associated with immune dysregulation

2025· article· en· W4416374040 on OpenAlexaffvenue
Andrew Wong-Pack, Vivian Szeto, Kristine Jeganathan, Mihir D. Bhatt, Rae Brager, Jenny Garkaby

Bibliographic record

VenueLymphoSign Journal · 2025
Typearticle
Languageen
FieldImmunology and Microbiology
TopicImmunodeficiency and Autoimmune Disorders
Canadian institutionsOntario Clinical Oncology GroupMcMaster UniversityChildren's Hospital of Eastern OntarioMcMaster University Medical Centre
Fundersnot available
KeywordsImmune dysregulationMissense mutationSanger sequencingImmune systemHaploinsufficiencyProbandCytotoxic T cell

Abstract

fetched live from OpenAlex

Background: Cytotoxic T lymphocyte-associated antigen-4 (CTLA4) haploinsufficiency is a primary immunoregulatory disorder characterized by a broad clinical spectrum including combined immunodeficiency, autoimmunity, enteropathy, lymphoproliferation, and malignancy. Aim: We aim to broaden the recognized phenotypic and genetic spectrum of patients with variants in CTLA4. Methods: We report the case of a patient with persistent bicytopenia and an episode of soft tissue infection and associated bacteremia. Targeted genetic sequencing was performed using next-generation sequencing on the Illumina platform with Sanger sequencing for low-coverage regions through Cincinnati Children’s Hospital in addition to immune evaluation. Results: The proband presented at 14 years of age with cellulitis, and was found to have Bacillus cereus bacteremia, along with neutropenia and thrombocytopenia that persisted following infection resolution. Immune evaluation revealed T cell lymphopenia with preserved humoral immunity, and subsequently NK cell lymphopenia. A targeted ALPS genetic panel identified a novel heterozygous variant in the CTLA4 NM_005214.5(CTLA4):c.160G>T (p.Ala54Ser). Conclusion: A novel heterozygous CTLA4 variant caused immune dysregulation and immunodeficiency, in keeping with the described features of CTLA4 haploinsufficiency. Statement of novelty: We describe a previously unreported missense variant in CTLA4 leading to immune dysregulation and cytopenias, successfully managed with targeted abatacept therapy.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Case report · Consensus signal: Case report
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.010

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0020.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0010.001
Science and technology studies0.0010.001
Scholarly communication0.0010.000
Open science0.0010.001
Research integrity0.0030.001
Insufficient payload (model declined to judge)0.0030.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.206
Teacher spread0.201 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designCase report
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes2
Has abstractyes

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