Regulation of feeding and metabolism by fat mass and obesity-associated protein in zebrafish
Bibliographic record
Abstract
The fat mass and obesity-associated (FTO) gene has been widely implicated in the regulation of energy balance in mammals. Whether FTO has similar roles in non-mammals remains unexplored. The goal of this research was to determine if FTO inhibition affects feeding, metabolism and lipid homeostasis in zebrafish (Danio rerio). Rhein, a pharmacological inhibitor of FTO was used to examine if FTO suppression modulates metabolic pathways in zebrafish liver and brain in vivo, and in zebrafish liver (ZFL) cells in vitro. Rhein treatment resulted in a significant reduction in food intake, accompanied by corresponding downregulation of FTO and its downstream effector IRX3 at both the mRNA and protein levels. Inhibiting FTO upregulated lipid oxidation genes and downregulated lipogenic pathways, indicating enhanced lipid catabolism. Moreover, rhein administration elevated key metabolic regulators AMPK and PPARα while suppressing gluconeogenic genes, suggesting improved glucose homeostasis. These findings suggest that FTO plays an important role in zebrafish metabolic regulation and that its inhibition modulates energy balance via the STAT3 signaling pathway. This research provides insights into the evolutionary conservation of FTO function and further supports FTO as a key molecule in zebrafish metabolic regulation.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".