Continuous light enhances adventitious rooting in Robinia pseudoacacia L. through a regulatory module involving RpCOP1 and auxin signaling
Bibliographic record
Abstract
Continuous light is a crucial environmental signal, yet its precise role in adventitious rooting in woody plants, particularly concerning the function of the classical light-signaling repressor CONSTITUTIVE PHOTOMORPHOGENIC 1, remains a significant open question. This study investigated how continuous light regulates adventitious root formation in Robinia pseudoacacia L. (black locust) through RpCOP1-mediated mechanisms. Black locust tissue culture plantlets were analyzed through anatomical observation, physiological measurements, hormone profiling, transcriptome sequencing with time-ordered gene co-expression network analysis, and heterologous overexpression validation in poplar. Continuous light treatment advanced rooting by 2 days, increasing adventitious root number by 86.03 % and root length by 110.76 %. The pro-adventitious rooting effect was associated with physiological reprogramming, wherein continuous light reduced photosynthetic efficiency while promoting soluble sugar accumulation at the stem base. Time-ordered gene co-expression network analysis identified RpCOP1 as a key upstream hub whose expression is induced by continuous light. Upregulation of RpCOP1 coincided with systemic hormonal reprogramming, highlighted by 60.72 % increase in auxin. Functional validation via heterologous overexpression of RpCOP1 in poplar supported this positive regulatory role. Compared with wild-type, transgenic lines under continuous light exhibited 33.33 % increase in adventitious root number and 75.85 % increase in root length, demonstrating a photoperiod-dependent effect, with rooting performance ordering continuous light > normal light > continuous dark. These findings support a context-dependent model where RpCOP1 function exhibits either repressive or activating roles depending on light environment and tissue type. • Time-ordered network identified RpCOP1 as key upstream regulator of rooting. • RpCOP1 positively regulates adventitious rooting under continuous light. • RpCOP1 shows conserved photoperiod-dependent regulation across woody species.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".