Conditioned Medium from R-Spondin1-Activated Human Umbilical Cord Mesenchymal Stem Cells Promotes Biological Functions of Human Umbilical Vein Endothelial Cells
Bibliographic record
Abstract
Abstract Wnt/β-catenin signaling pathway is an important cell-cell communication system, which plays an indispensable role in skin wound repair by stimulating cell proliferation, dedifferentiation and tissue remodeling. Meanwhile, R-spondin1 (RSPO1), as a classic Wnt agonist, can significantly enhance the activity of the Wnt/β-catenin signaling pathway. Human umbilical cord mesenchymal stem cells (UCMSC) are regulatory cells that accelerate wound healing primarily through the release of paracrine signaling molecules, but the underlying effects of RSPO1-activated UCMSC-conditioned medium (UCMSC-CM) on skin wound repair have not been clearly defined. In our study, we investigated the role of RSPO1-activated UCMSC-conditioned medium on the biological activities of human umbilical vein endothelial cells (HUVECs), which are widely used as a model for studying angiogenesis related to wound healing. After determining the optimal action condition of RSPO1 on UCMSC and the protein concentration of RSPO1-activated UCMSC-CM on HUVECs by the Cell Counting Kit-8 (CCK-8) method, we comprehensively evaluated the effect of RSPO1-activated UCMSC-CM on the biological functions of HUVECs by BrdU proliferation assay, tubule formation assay and scratch assay, in order to provide new therapeutic ideas for skin wound healing. Our findings revealed that, in comparison to the non-RSPO1-activated control group, RSPO1-activated UCMSC-CM exhibited more robust stimulatory effects on HUVEC proliferation, angiogenesis, and cell migration. This suggests that targeting RSPO1-activated UCMSC-CM may represent a promising strategy for enhancing wound healing outcomes.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".