Characterization, Production, and Application of Antifungal Metabolites from Probiotic Levilactobacillus and Lactiplantibacillus Strains Isolated from Fermented Olives
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
In this study, the characterization, production, and application of antifungal metabolites obtained from the Levilactobacillus (L. brevis S27) and two Lactiplantibacillus (L. pentosus S42 and L. plantarum S62) strains were evaluated. The lactic acid bacteria (LAB) cells showed antifungal activity against molds. The cell-free supernatant (CFS) of Levilactobacillus and Lactiplantibacillus presented antimicrobial activity against fungi and foodborne pathogenic bacteria, and the antifungal activity was significantly (p < 0.05) higher than that of the antibacterial activity. Moreover, the antifungal metabolites were characterized as proteinaceous compounds that remained stable under both high and low temperatures and demonstrated activity across a broad pH range. Additionally, metabolite production was significantly higher (p < 0.05) at an initial pH of 5 when incubated at either 25 °C or 37 °C. All strains and their CFSs exhibited strong bio-preservative effects against Penicillium digitatum in yogurt and against Aspergillus niger on orange fruit. Consequently, these Lactobacilli strains and their antifungal metabolites represent a novel approach to biocontrol in the agri-food industry and agricultural products.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it