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Record W4416771670 · doi:10.26434/chemrxiv-2025-sfgms

From Computer to Cognition: Synthesis and Preclinical Validation of a Naphthalene Amide as a Novel Anti-Alzheimer Agent

2025· preprint· W4416771670 on OpenAlexafffund
Saniya Fatima, Fareeha Anwar, Uzma Saleem, Bashir Ahmad, Lee Yean Kee, Iskandar Abdullah, Muhammad Usman Mirza, John F. Trant, Sarfraz Ahmad

Bibliographic record

VenueChemRxiv · 2025
Typepreprint
Language
FieldMedicine
TopicCholinesterase and Neurodegenerative Diseases
Canadian institutionsUniversity of Windsor
FundersRiphah International UniversityNatural Sciences and Engineering Research Council of CanadaUniversiti Malaya
KeywordsIn vivoIn vitroOxidative stressAchéIn silicoAcetylcholinesteraseNootropicIn vitro toxicology

Abstract

fetched live from OpenAlex

Alzheimer's disease is a major global health challenge, imposing a significant healthcare burden due to irreversible neuronal degeneration and impaired cognitive function. An extensive in silico screening followed by in vitro testing was performed to identify potential cost-effective AChE inhibitors for AD treatment. The newly identified compound (SF2) was synthesized and investigated for in vitro and in vivo activities and toxicities. In vitro activity was assessed using AChE inhibitory potential, and toxicity was evaluated using MTT assay on mouse-brain capillary endothelial cells. For in vivo studies, mice were divided into seven groups and treated with SF2 at four different doses for 23 days. Visuospatial learning and memory retention was determined with the help of four nootropic models. The protective effect of SF2 against the AD was assessed by the levels of SOD, CAT, GSH, Nitrite, MDA, amyloid-β, tau, TNF-α, and IL-6. Selected organs (brain, kidney, liver, heart and spleen) were investigated for their oxidative stress markers, along-with CBC, liver and renal function tests. Pronounced amelioration in cognitive performance was observed by SF2 treatment. It significantly attenuated changes in brain pathology, acetylcholinesterase activity, and inflammatory markers induced by the streptozotocin.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.010

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0030.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.104
GPT teacher head0.368
Teacher spread0.264 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes2
Has abstractyes

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