Percutaneous Intramyocardial Septal Ablation with Nanosecond Pulsed Electric Fields in Canine Model
Bibliographic record
Abstract
Background: Nanosecond pulsed electric field (nsPEF) is a nonthermal ablation technique that utilizes ultra-rapid electrical pulses to induce selective cell death with minimal inflammatory response. The objective of this study was to evaluate the safety and efficacy of percutaneous intramyocardial septal nsPEF ablation (PIMSNA) for septal reduction therapy (SRT) in a canine model (Labrador dogs). Methods: The acute and chronic effects of this novel PIMSNA approach under transthoracic echocardiography (TTE) guidance were comprehensively assessed. The optimal ablation parameters for inducing the targeted myocardial pathological process were selected. The major adverse events monitored included post-ablation endocardial regional edema, arrhythmias (e.g., ventricular tachycardia, ventricular fibrillation, atrioventricular conduction block), pericardial effusion, and cardiac tamponade. The assessment of acute and chronic effects was conducted over a 90-day follow-up period using serial TTE imaging, electrocardiographic parameters, blood biochemistry analyses, and histopathological evaluation. The intervention was performed on Labrador canines. Results: The experimental findings demonstrated that an applied voltage of 3,000 volts yielded the most effective ablation outcome. TTE-guided PIMSNA procedures resulted in a 10% impedance decline within the targeted region, indicative of successful ablation. The motion amplitude and systolic wall thickening rate demonstrated a substantial decrease and remained persistently low in the ablated septal region. However, septal thickness exhibited no substantial alterations subsequent to PIMSNA. Histological examination confirmed the presence of cardiomyocyte death, characterized by progressive cytoplasmic hyperchromasia and nuclear pyknosis. A notable observation is the minimal presence of acute-phase inflammatory cell infiltration. There were no instances of sustained ventricular tachycardia, ventricular fibrillation, or atrioventricular conduction block during PIMSNA. Conclusion: The novel PIMSNA technique effectively induced myocardial injury and regional hypokinesis without significant acute edema. Histopathological analysis revealed significant programmed cell death of cardiomyocytes accompanied by minimal inflammatory cell infiltration.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".