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Percutaneous Intramyocardial Septal Ablation with Nanosecond Pulsed Electric Fields in Canine Model

2025· preprint· W4416776282 on OpenAlex
Zhihong Zhao, Xiangyang Xia, Jing Li, Yonggang Chen, Gaodong Qiu, Siying Liang, Xinping Lin, Bin Wu, Xinhua Chen, Zhaoxia Pu

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

aboutThe title or abstract carries a Canadian signal from the geographic lexicon.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenuePreprints.org · 2025
Typepreprint
Language
FieldBiochemistry, Genetics and Molecular Biology
TopicMicrobial Inactivation Methods
Canadian institutionsnot available
FundersZhejiang Medical Association
KeywordsAblationPercutaneousAtrioventricular blockElectrical conduction system of the heartCardiac AblationVentricular fibrillationCatheter ablation

Abstract

fetched live from OpenAlex

Background: Nanosecond pulsed electric field (nsPEF) is a nonthermal ablation technique that utilizes ultra-rapid electrical pulses to induce selective cell death with minimal inflammatory response. The objective of this study was to evaluate the safety and efficacy of percutaneous intramyocardial septal nsPEF ablation (PIMSNA) for septal reduction therapy (SRT) in a canine model (Labrador dogs). Methods: The acute and chronic effects of this novel PIMSNA approach under transthoracic echocardiography (TTE) guidance were comprehensively assessed. The optimal ablation parameters for inducing the targeted myocardial pathological process were selected. The major adverse events monitored included post-ablation endocardial regional edema, arrhythmias (e.g., ventricular tachycardia, ventricular fibrillation, atrioventricular conduction block), pericardial effusion, and cardiac tamponade. The assessment of acute and chronic effects was conducted over a 90-day follow-up period using serial TTE imaging, electrocardiographic parameters, blood biochemistry analyses, and histopathological evaluation. The intervention was performed on Labrador canines. Results: The experimental findings demonstrated that an applied voltage of 3,000 volts yielded the most effective ablation outcome. TTE-guided PIMSNA procedures resulted in a 10% impedance decline within the targeted region, indicative of successful ablation. The motion amplitude and systolic wall thickening rate demonstrated a substantial decrease and remained persistently low in the ablated septal region. However, septal thickness exhibited no substantial alterations subsequent to PIMSNA. Histological examination confirmed the presence of cardiomyocyte death, characterized by progressive cytoplasmic hyperchromasia and nuclear pyknosis. A notable observation is the minimal presence of acute-phase inflammatory cell infiltration. There were no instances of sustained ventricular tachycardia, ventricular fibrillation, or atrioventricular conduction block during PIMSNA. Conclusion: The novel PIMSNA technique effectively induced myocardial injury and regional hypokinesis without significant acute edema. Histopathological analysis revealed significant programmed cell death of cardiomyocytes accompanied by minimal inflammatory cell infiltration.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Research integrity
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.152
Threshold uncertainty score0.999

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.001
Research integrity0.0020.002
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.045
GPT teacher head0.331
Teacher spread0.286 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it