Targeted silencing of exopolysaccharide biosynthesis genes via CRISPR/dCas9 to enhance polyhydroxybutyrate production from biodiesel-derived crude glycerol in Burkholderia sp.
Bibliographic record
Abstract
The newly isolated Burkholderia sp. SCN-KJ, which is capable of simultaneously producing intracellular polyhydroxybutyrate (PHB) and exopolysaccharide (EPS) from biodiesel-derived crude glycerol, presented a significant challenge in this study because of interference from EPS synthesis. The bceA gene, which encodes a bifunctional protein responsible for mannose-6-phosphate isomerization and the mannose-1-phosphate guanylyltransferase reaction, was identified as a key regulator of EPS production. To address this issue, CRISPR interference (CRISPRi) was employed to target the bceA gene, leading to the complete suppression of EPS synthesis and a significant improvement in PHB yield. Under optimized culture conditions, the ΔbceA mutant strain exhibited complete inhibition of EPS production, resulting in a 1.75-fold increase in the PHB concentration and PHB of a higher molecular weight than that of the wild-type Burkholderia sp. SCN-KJ. These results highlight the effectiveness of dCas9-mediated silencing of the bceA gene in overcoming the challenges posed by EPS interference and underscore its potential for increasing PHB production in Burkholderia sp. SCN-KJ.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.004 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".