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Record W4416980514 · doi:10.26434/chemrxiv-2025-tm1ms

The need for standardisation and improved open (meta)data practices in metaproteomics

2025· article· W4416980514 on OpenAlexaff
Tim Van Den Bossche, Maximilian Wolf, Jean Armengaud, Magnus Ø. Arntzen, Dirk Benndorf, Daniel Figeys, Lucia Grenga, Robert L. Hettich, Nithu Sara John, Pratik Jagtap, Nico Jehmlich, Manuel Kleiner, Benoît J. Kunath, Leyuan Li, Mary Lipton, Bart Mesuere, Benjamin A. Neely, Zhibin Ning, Yasset Pérez‐Riverol, Jeena Rajan, Kay Schallert, Jana Seifert, Sergio Uzzau, Pieter Verschaffelt, Paul Wilmes, Juan Antonio Vizcaíno, Lennart Martens, Robert Heyer

Bibliographic record

VenueChemRxiv · 2025
Typearticle
Language
FieldChemistry
TopicAdvanced Proteomics Techniques and Applications
Canadian institutionsUniversity of Ottawa
Fundersnot available
KeywordsMetaproteomicsInteroperabilityWorkflowMetadataStandardizationData curationAutomationLimiting

Abstract

fetched live from OpenAlex

Background: Metaproteomics enables functional insight into microbial communities by identifying and quantifying proteins in complex samples. Yet, heterogeneous analytical workflows and the lack of standardization across experimental and bioinformatic stages hinder reproducibility and comparability, limiting integration with other omics data. Results: We here present a community-developed reporting checklist tailored to the specific needs of metaproteomics. We also outline current efforts to enable structured and interoperable metadata capture, drawing on standards from proteomics and microbiome research wherever possible. Conclusions: By promoting transparent reporting and advancing metadata practices, our recommendations aim to align metaproteomics more closely with FAIR principles and support reproducible and interoperable research practices.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.619
metaresearch head score (Gemma)0.609
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesMetaresearch, Open science
Consensus categoriesMetaresearch
DomainCandidate signal: Reproducibility · Consensus signal: none
Study designCandidate signal: Theoretical or conceptual · Consensus signal: none
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.984
Threshold uncertainty score0.470

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.6190.609
Meta-epidemiology (narrow)0.0030.003
Meta-epidemiology (broad)0.0030.005
Bibliometrics0.0160.014
Science and technology studies0.0060.016
Scholarly communication0.0270.052
Open science0.0160.029
Research integrity0.0090.028
Insufficient payload (model declined to judge)0.0020.003

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.085
GPT teacher head0.400
Teacher spread0.315 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; the direct Gemma label and the distilled Codex classifier agree on what is shown here.

Study designTheoretical or conceptual
DomainReproducibility
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2025
Admission routes1
Has abstractyes

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