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Record W4416994897 · doi:10.3389/fnimg.2025.1637148

Understanding false positives in control conditions: a simulation study of post-hoc testing in low-powered neuroimaging trials

2025· article· en· W4416994897 on OpenAlexaff
Ji Hyun Ko

Bibliographic record

VenueFrontiers in Neuroimaging · 2025
Typearticle
Languageen
FieldNeuroscience
TopicFunctional Brain Connectivity Studies
Canadian institutionsUniversity of ManitobaHealth Sciences Centre
Fundersnot available
KeywordsBlindingNeuroimagingFalse positive paradoxRelevance (law)False positives and false negativesPsychological interventionRandomized controlled trialControl (management)Sample size determination

Abstract

fetched live from OpenAlex

Randomized controlled trials (RCTs) are essential for evaluating treatment efficacy, typically comparing active interventions to control conditions. In situations where blinding is impractical—such as in psychological therapies or physical rehabilitation—waitlist controls are often used to account for natural symptom progression and test–retest variability. This study examines the biases introduced by post-hoc analyses under conditions of low statistical power, particularly in neuroimaging research. Through large-scale simulations involving 100 million datasets with varying sample sizes, treatment effects, and test–retest variability, the study demonstrates that the common practice of conducting post-hoc tests only on brain regions showing significant interaction effects can substantially increase the false positive rate in the control condition. These findings underscore the relevance of Berkson’s paradox in interpreting unexpected control group outcomes and caution against overinterpreting such results. A complementary neuroimaging simulation reinforces these conclusions, emphasizing the need for critical scrutiny when evaluating significant effects in control groups. Overall, this work challenges conventional post-hoc testing strategies and advocates for a more nuanced and statistically informed interpretation of results, especially in studies with limited power.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.054
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMetaresearch, Meta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.264
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0020.054
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0020.002
Science and technology studies0.0000.000
Scholarly communication0.0000.001
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.125
GPT teacher head0.345
Teacher spread0.221 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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