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Record W4417013791 · doi:10.1016/j.jacig.2025.100622

High levels of nonblocking anti-interferon and anticytokine autoantibodies in individuals with mRNA vaccine–induced systemic allergic reactions

2025· article· en· W4417013791 on OpenAlexaff
Müge Kalaycıoğlu, Allan Feng, Shaurya Dhingra, Muhammad Bilal Khalid, Kari C. Nadeau, Paul J. Utz, Pamela A. Frischmeyer‐Guerrerio

Bibliographic record

VenueJournal of Allergy and Clinical Immunology Global · 2025
Typearticle
Languageen
FieldMedicine
TopicDrug-Induced Adverse Reactions
Canadian institutionsInstitute of Infection and Immunity
FundersNational Institute of Allergy and Infectious DiseasesNHLBI Division of Intramural ResearchNational Institutes of Health
KeywordsAutoantibodyMessenger RNAImmune systemAdverse effectAntibodyAllergy

Abstract

fetched live from OpenAlex

Background: Hypersensitivity reactions following coronavirus 2019 disease (COVID-19) mRNA vaccination, although rare, have raised public concern and contributed to vaccine hesitancy. The underlying mechanisms and cofactors that increase the risk of these adverse reactions remain poorly understood. Objective: We aimed to investigate whether the presence of autoantibodies, particularly anticytokine autoantibodies (ACAs), correlates with systemic adverse events (SAEs) in response to mRNA COVID-19 vaccines. Methods: We analyzed serum samples from 2 independent cohorts of individuals who experienced convincing SAEs after receiving their first dose of COVID-19 mRNA vaccine, including 16 individuals at the National Institutes of Health (NIH) and 18 at Stanford University. Individuals enrolled in the NIH cohort received subsequent vaccine doses under medical supervision. The control groups included vaccine-tolerant individuals. Bead-based autoantigen arrays were used to detect autoantibodies, whereas cell-based assays were used to assess the functional blocking activity of specific antibodies. Results: Autoantibody positivity was detected in 81.2% of the NIH cohort and 38.8% of the Stanford cohort. Elevated levels of antibodies against IFN-λ1 were associated with repeated SAEs in the NIH cohort. Other notable targets included IL-1A, IL-4, IL-6, IL-11, IL-17, TNF-α, and IFN-γ. Despite elevated autoantibody levels, functional blocking activity was not detected in reporter assays. Conclusion: Our findings reveal a potential link between cytokine-targeting autoantibodies, especially anti-IFN-λ1, and systemic adverse responses to mRNA vaccination. These results suggest a role for immune dysregulation in individuals with hypersensitivity to mRNA vaccines and highlight the need for further investigation to improve vaccine safety and tolerance.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.113
Threshold uncertainty score0.652

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.027
GPT teacher head0.341
Teacher spread0.314 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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