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Record W4417015526 · doi:10.5376/lgg.2025.16.0015

Application of Single-Cell RNA-Seq in Legume Root Development Studies

2025· article· W4417015526 on OpenAlex
Hongpeng Wang, Wenxia Wu

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

venuePublished in a venue whose home country is Canada.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenueLegume Genomics and Genetics · 2025
Typearticle
Language
FieldAgricultural and Biological Sciences
TopicLegume Nitrogen Fixing Symbiosis
Canadian institutionsnot available
Fundersnot available
KeywordsLegumeRoot (linguistics)Adaptation (eye)AgriculturePlant developmentArabidopsisPlant tissueResearch development

Abstract

fetched live from OpenAlex

The single-cell RNA sequencing method known as scRNA-seq has revolutionized the study of plant root cellular complexity and developmental processes. The research combines modern scRNA-seq methods to study legume root development by showing how these methods help identify cell types and track cell lineages and detect short-lived cell populations. The research in Arabidopsis model systems produced complete root cell maps which revealed vital elements that regulate cell development and environmental adaptation thus enabling legume research. ScRNA-seq analysis of legumes has enabled researchers to study gene expression patterns in different cell types during root and nodule development which has enhanced our understanding of symbiotic processes and plant stress mechanisms. Single-cell plant research encounters technical barriers because of protoplasting artifacts and cell capture biases but scientists continue to develop their research through the combination of single-nucleus RNA-seq and spatial transcriptomics. The research investigates the necessity of root cell atlases that span multiple species within legumes while exploring the combination of scRNA-seq with other omics methods and their potential to develop new crop improvement approaches based on single-cell research findings. ScRNA-seq technology has the potential to revolutionize our knowledge of legume root biology which will drive major breakthroughs in plant developmental research and sustainable agricultural methods.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.168
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.001
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.020
GPT teacher head0.236
Teacher spread0.216 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it