Complete Genome Sequence of Pithoascus kurdistanensis CBS 149789, an Endophytic Fungus Isolated from Papaver bracteatum
Bibliographic record
Abstract
Endophytic fungi are increasingly recognized as pivotal contributors to plant secondary metabolism, often synthesizing bioactive compounds like those produced by their hosts. We report the first complete genome sequence for Pithoascus kurdistanensis, a novel endophyte isolated from Papaver bracteatum that produces morphinan alkaloids independently from its host plant. High-quality genomic DNA from P. kurdistanensis was subjected to a hybrid sequencing strategy using both Oxford Nanopore long-read and Illumina short-read platforms, yielding a 34.0 Mbp assembly composed of nine chromosomal contigs and four additional minor contigs. This assembly was 97.3% complete as determined by BUSCO and revealed that 6.37% of the genome consists of repetitive elements. A total of 8292 protein-coding genes, including 63 candidate genes potentially involved in isoquinoline alkaloid biosynthesis, have been identified. Phylogenetic analysis based on conserved single-copy orthologs positioned P. kurdistanensis within a basal lineage of the Microascaceae. Overall, these results provide foundational insight into the genetic potential of P. kurdistanensis as a novel microbial source of morphinan alkaloids, while emphasizing the need for continued functional studies to resolve the underlying biosynthetic pathways.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".