Phosphate Resupply Differentially Impacts the Shoot and Root Proteomes of <i>Arabidopsis thaliana</i> Seedlings
Bibliographic record
Abstract
Phosphate (Pi) is an essential macronutrient for plant development that is often limited in soil. Plants have evolved dynamic biochemical, physiological and morphological adaptations to cope with Pi deficiency, known as the Pi starvation response (PSR). While many components of the PSR have been well-characterised, less is known about how metabolic homoeostasis is re-established upon Pi resupply, particularly tissue- and time-specific adaptations. Here, we applied label-free quantitative proteomics to quantify protein-level changes in Arabidopsis thaliana shoots and roots following Pi resupply after prolonged Pi deprivation. Sampling at 1 h and 48 h time-points, we captured immediate signalling and metabolic responses, along with longer-term recovery processes. Early responses prioritised metabolic adjustments restoring Pi pools via enhanced glycolysis and energy production, followed by later shifts toward anabolism. Several key enzymes, including ALTERNATIVE OXIDASE 1 A, FRUCTOSE-BISPHOSPHATE ALDOLASE 5 and subunits of PHOTOSYSTEM I exhibited tissue-specific and time-dependent regulation. Our findings reveal dynamic phases of metabolic reprogramming during recovery from Pi starvation, and identify candidate proteins as potential targets for enhancing Pi uptake- and use-efficiency in crops. While hydroponic liquid culture enabled precise control of Pi availability, soil responses may be further influenced by heterogeneity and other root interactions.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".