MétaCan
Menu
Back to cohort
Record W4417421174 · doi:10.1021/jacs.5c11066

Bridging a Gap in Marine Sulfur Cycling: Discovery of a <scp>d</scp> -Cysteinolic Acid Degradation Pathway

2025· article· en· W4417421174 on OpenAlexaff
Laura Burchill, Adam W. E. Stewart, Luise Pallasdies, Mihwa Lee, Lisa S. Y. Coe, Luca Zudich, Leila Jebeli, Mahima Sharma, Vinzenz Hofferek, Malcolm J. McConville, G.J. Davies, Nichollas E. Scott, Bryndan P. Durham, Spencer J. Williams

Bibliographic record

VenueJournal of the American Chemical Society · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicNitrogen and Sulfur Effects on Brassica
Canadian institutionsInstitute of Genetics
FundersNational Health and Medical Research CouncilAustralian Research CouncilUniversity of MelbourneAustralian Academy of ScienceRoyal SocietyBiotechnology and Biological Sciences Research CouncilDirectorate for STEM EducationUniversity of FloridaSimons Foundation
KeywordsRoseobacterMetabolic pathwayBacteriaMarine bacteriophageSulfurArchaeaChloroplastPhotosynthesis

Abstract

fetched live from OpenAlex

d -Cysteinolic acid is a prominent sulfur-containing compound in marine and freshwater ecosystems, yet its metabolic fate has remained largely uncharacterized. Here, we describe a newly identified d -cysteinolic acid degradation pathway in a marine model bacterium Ruegeria pomeroyi DSS-3. This pathway involves a PLP-dependent cysteinolic acid racemase (ClaA) that interconverts d - and l -cysteinolic acids, followed by a NAD + -dependent l -cysteinolic acid dehydrogenase (ClaB) that oxidizes l -cysteinolic acid to l -cysteate. A cysteate racemase (CuyB) then converts l -cysteate to d -cysteate, the preferred substrate for the sulfolyase CuyA. Bioinformatic analysis of the Tara Oceans gene atlas reveals that ClaA and CuyA homologues are widespread in marine bacterial populations, particularly within the alphaproteobacterial Roseobacter and SAR116 groups and the gammaproteobacterial SUP05 clade. Relative abundance of gene homologues correlates with surface water chlorophyll levels, linking d -cysteinolic acid degradation to photosynthetic primary production. This discovery advances our understanding of marine sulfur cycling and highlights d -cysteinolic acid as an important metabolic currency in microbial networks.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.007
GPT teacher head0.241
Teacher spread0.234 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

Explore more

Same venueJournal of the American Chemical SocietySame topicNitrogen and Sulfur Effects on BrassicaFrench-language works237,207