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Biodegradability and Ecotoxicity Profiles of Choline Acetate, Betaine, and L-Proline NADESs: A Hidden Threat for Eutrophication?

2025· preprint· W4417445554 on OpenAlexfundno aff
Nandish M Nagappa, Angelica Mero, Elena Husanu, Zeba Usmani, Matteo Oliva, Matilde Vieira Sanches, Giorgia Fumagalli, Andrea Mele, Andrea Mezzetta, Nicholas Gathergood, Lorenzo Guazzelli, Carlo Pretti, Yevgen Karpichev

Bibliographic record

VenuePreprints.org · 2025
Typepreprint
Language
FieldEnvironmental Science
TopicChemistry and Chemical Engineering
Canadian institutionsnot available
FundersMinistry of Rural Affairs
KeywordsBiodegradationEcotoxicityGreen chemistryEutectic systemSolventBottleSAFERCholine chloride

Abstract

fetched live from OpenAlex

Deep Eutectic Solvents (DESs) and in essence naturally available DESs (NADESs) are considered to be green solvents due to their low vapor pressure, non-flammability, thermal stability, good solvent power and low oxicity. These properties make them attractive as safer and more environmentally acceptable solvent options. Green Chemistry promotes the use of renewable and biocompatible compounds such as amino acids, lipids and acids of natural origin to yield more sustainable DESs, which yields their application in several industrial processes. Driven by the current requisite for sustainable progress, along with overcoming dependence on fossil-based resources, the current work details important findings pertaining to the design of sustainable NADESs from the perspective of green chemistry to exhibit suitable physico-chemical properties and a low toxicological profile. Biodegradation studies using OECD 301D closed bottle test (CBT) were performed to observe the biodegradability of 15 selected NADESs. Toxicity controls were run along with the CBT run to observe the behavior of these NADESs in the environment. In this framework, the present paper investigates the development of safer NADESs. The results obtained suggest that our synthesized NADESs, have high biodegradability and low toxicity towards microalgae. Although a conventional threat to the environment would seem out of reach, it must be hypothesized that such compounds might act as enhancers of eutrophication phenomena.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.051
GPT teacher head0.311
Teacher spread0.259 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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Same venuePreprints.orgSame topicChemistry and Chemical EngineeringFrench-language works237,207