Oncostatin M (OSM) Mediated Alterations in Matrix Metalloproteinase (MMP) ‐ 2 is Regulated by Specific Signal Transduction Pathways in H‐ras Transformed Cells
Bibliographic record
Abstract
Oncostatin M (OSM) is a pleiotropic cytokine that has been shown to exert both stimulatory and inhibitory effects on tissue invasion in various cell lines. Tissue invasion is a key marker of metastasis and can be monitored by examining matrix metalloproteinase (MMP) activity. OSM has been shown to up‐regulate MMP‐2 protein expression and this regulation may involve the JAK‐STAT pathway for signal transduction. This present study elucidates a link between OSM, MMP‐2 protein expression and specific signal transduction pathways in NR3 cells (H‐ras transformed fibroblasts capable of benign tumor formation). The mitogen activated protein kinase (MAPK) pathway and the protein kinase C (PKC) pathway are involved in the OSM mediated alterations in MMP‐2 protein expression in NR3 cells. The P‐I‐3 kinase pathway and the AKT pathway are apparently not involved in this OSM mediated signaling mechanism in NR3 cells. Treatment of NR3 cells with OSM also resulted in the activation of specific members of the JAK‐STAT signal transduction pathway. Protein expression levels of JAK 1,2,3 and STAT 1,2,3,4 in NR3 cells were determined by Western blot analysis following treatment of NR3 cells with 50 ng/ml OSM for 24 hr. JAK1,2,3, STAT‐2, and STAT‐3 were involved in NR3's MMP‐2 response to OSM but STAT‐1 and STAT‐4 showed no apparent involvement in the signaling pathway [N.S.E.R.C. (R.A.R.H) and Canadian Cancer Society (P.E.I. Division)].
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".