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Oncostatin M (OSM) Mediated Alterations in Matrix Metalloproteinase (MMP) ‐ 2 is Regulated by Specific Signal Transduction Pathways in H‐ras Transformed Cells

2008· article· en· W52987491 on OpenAlexaffabout
Eddie Joesph Francis, Robert A. R. Hurta

Bibliographic record

VenueThe FASEB Journal · 2008
Typearticle
Languageen
FieldMedicine
TopicCytokine Signaling Pathways and Interactions
Canadian institutionsUniversity of Prince Edward Island
Fundersnot available
KeywordsOncostatin MSignal transductionCell biologyJAK-STAT signaling pathwayBiologyProtein kinase BProtein kinase AKinaseChemistryCancer researchCytokineInterleukin 6Tyrosine kinaseImmunology

Abstract

fetched live from OpenAlex

Oncostatin M (OSM) is a pleiotropic cytokine that has been shown to exert both stimulatory and inhibitory effects on tissue invasion in various cell lines. Tissue invasion is a key marker of metastasis and can be monitored by examining matrix metalloproteinase (MMP) activity. OSM has been shown to up‐regulate MMP‐2 protein expression and this regulation may involve the JAK‐STAT pathway for signal transduction. This present study elucidates a link between OSM, MMP‐2 protein expression and specific signal transduction pathways in NR3 cells (H‐ras transformed fibroblasts capable of benign tumor formation). The mitogen activated protein kinase (MAPK) pathway and the protein kinase C (PKC) pathway are involved in the OSM mediated alterations in MMP‐2 protein expression in NR3 cells. The P‐I‐3 kinase pathway and the AKT pathway are apparently not involved in this OSM mediated signaling mechanism in NR3 cells. Treatment of NR3 cells with OSM also resulted in the activation of specific members of the JAK‐STAT signal transduction pathway. Protein expression levels of JAK 1,2,3 and STAT 1,2,3,4 in NR3 cells were determined by Western blot analysis following treatment of NR3 cells with 50 ng/ml OSM for 24 hr. JAK1,2,3, STAT‐2, and STAT‐3 were involved in NR3's MMP‐2 response to OSM but STAT‐1 and STAT‐4 showed no apparent involvement in the signaling pathway [N.S.E.R.C. (R.A.R.H) and Canadian Cancer Society (P.E.I. Division)].

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.005
Threshold uncertainty score0.997

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0040.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.030
GPT teacher head0.260
Teacher spread0.230 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2008
Admission routes2
Has abstractyes

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