Human Neutrophils produce alternatively spliced variants of 5‐Lipoxygenase mRNA
Bibliographic record
Abstract
5-lipoxygenase (5-LO) catalyzes the oxygenation of the 5th carbon on arachidonic acid and is, therefore, an essential enzyme for leukotriene biosynthesis. Activation of the 5-LO pathway following the stimulation of human neutrophils (PMN) leads to the formation of LTB4, a potent chemoattractant involved in the inflammatory response. Our studies focus on the mechanisms by which the biosynthesis of 5-LO products is controlled. In this study, we evaluated whether alternative forms of 5-LO mRNA exist in human PMN. Following reverse transcription and PCR analyses of total mRNA extracted from PMN isolated from peripheral blood, several potential variants of 5-LO mRNA were identified. Molecular cloning and sequencing experiments revealed that at least two isoforms are present in human PMN: one containing all 14 exons with the expected splicing sites and one that results from the inclusion of intron 10. Approximately 20% of generated clones contained this variant isoform. Treatment of human PMN with granulocyte-macrophage colony stimulating factor (GM-CSF) did not result in the appearance of new isoforms. The retention of intron 10 in the mRNA produces a frame shift in the open reading frame and the immediate appearance of the stop codon TGA. Experiments are underway to determine whether the translation product of the alternative isoform impacts on the capacity for the biosynthesis of 5-LO products. (Supported by the Canadian Institutes of Health Research and the Canada Research Chairs Program)
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".