Relative Expression of Anti-Oxidant Genes in Adult Human Progenitor and Mature Oligodendrocytes (S12.005)
Bibliographic record
Abstract
OBJECTIVE:To compare expression of anti-oxidant related genes in mature oligodendrocytes(OLs) with their progenitors (OPCs) using cells isolated from adult non-inflamed CNS tissue samples. BACKGROUND:Oxidative stress is implicated as contributing to injury to OLs in multiple sclerosis lesions. Coincident injury of OPCs in such lesions could impact on potential for tissue repair. Previous in vitro studies show that human adult CNS derived OPCs are more vulnerable to stress induced injury than are mature cells and that in MS lesions there is a relatively greater loss of OPCs than OLs (Cui 2013). DESIGN/METHODS:Cell populations were isolated from surgically resected temporal lobe tissue samples using an initial trypsin/Percoll gradient step followed by a 2-day in vitro micro-titre plate adhesion step to remove microglia. OLs (A2B5- fraction) and OPCs (A2B5+ fraction) were then isolated using A2B5 antibody coated immune-magnetic beads. Aliquots of 3 separate samples were then collected in Trizol. Subsequently RNA was extracted and subjected to Affymetrix based microarrays. Anti-oxidant gene analysis was performed using Partek-6 genomic suite. RESULTS:Previous analysis had indicated overexpression of OPC vs. mature OL lineage genes as well as over-expression of external apoptotic pathway genes in the A2B5+ fraction (Cui 2013). Oxidative stress related genes more highly expressed in the A2B5- under the basal conditions included Super Oxide Dismutase 3 (SOD3), Glutathione S transferase alpha 5 (GSTA5), Glutathione S transferase alpha 1 (GSTA1), Dual Oxidase 1 (DUO1), Dual Oxidase 2 (DUO2), Eosinophil Peroxidase (EPX), Myeloperoxidase (MPO), Prostaglandin Enteroperoxide Synthase 1 (PTGS1), Metallothionen 1H (MT1H), Thioredoxin Reducatse 3 N (TXNRD3NB). Mean up-regulation values ranged for 1.4 - 1.8 (p<.05). CONCLUSIONS:The microarray results suggest that mature human OLs express a basal level of anti-oxidant genes that may contribute to their relative in- situ and in vitro resistance to stress compared to OPCs. Study Supported by: Multiple Sclerosis Society of Canada
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".