The role of the Paf1 complex in controlling transcriptioncoupled histone modifications
Bibliographic record
Abstract
Histone modifications that are deposited during transcription elongation impact the recruitment and activities of proteins that further restructure the chromatin template. The Paf1 complex (Paf1C) functions at the intersection of transcription elongation and histone modification by associating with RNA pol II and facilitating the modification of histones. We are exploring three aspects of Paf1C function in yeast: the mechanism by which it stimulates histone H2B K123 ubiquitylation, the nature of its physical interaction with RNA pol II, and its involvement in transcription termination. We have recently described a small domain within the Rtf1 subunit of Paf1C that is necessary and sufficient for promoting H2B ubiquitylation. Using a multidisciplinary approach, we are currently investigating how this histone modification domain interacts with chromatin and stimulates histone modification. In a separate study, we have recently identified a domain within Rtf1 that tethers Paf1C to RNA pol II through an interaction with the elongation factor Spt5. This interaction ensures that the diverse functions of Paf1C are properly targeted to active genes. Finally, using high‐resolution tiling array studies, we have demonstrated broad and gene‐specific effects of Paf1C on the termination of snoRNAs, revealing genes that differ in their dependence on Rtf1‐mediated histone modifications. Funded by NIH and CIHR.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".