Evaluation of Inhibitory Activities of Different Chinese Herbal Medicine Plant Extracts Against Banana Fusarium Wilt Disease
Bibliographic record
Abstract
【Objective】The research aims to investigate the inhibitory effects of different Chinese herbal medicine extracts on banana Fusarium wilt, and to screen effective plant resources for the control of banana Fusarium wilt.【Method】Tests were carried out on 29 Chinese herbal medicines, including Rheum palmatum and Andrographis paniculata, and the effective active ingredients were extracted by ethanol extraction. The inhibitory effects of different extracts on Foc TR4 were detected by the growth rate method, and the ethanol extracts of Rheum palmatum were extracted by using four solvents with different polarities, namely, petroleum ether, chloroform, ethyl acetate and n-butanol. The inhibitory effects of the extracts on the spores germination of banana Fusarium wilt were determined by the pendant drop method, and the effects of the extracts on the mycelial morphology of banana Fusarium wilt were observed.【Result】The inhibitory effects of 29 herbal medicine extracts on Foc TR4 showed great differences, and four extracts, including Rheum palmatum, Andrographis paniculata, Sophora flavescens, and Chrysanthemum indicum had better inhibitory effects on Foc TR4, among which the best inhibitory effect was achieved by Rheum palmatum extract, with the inhibitory rate of 6 mg/mL Rheum palmatum extract being 48.57%, and the EC50 of the mycelial growth being 6.68 mg/mL, while the EC50 of the spore germination was 4.60 mg/mL. Through observation by light microscope, the Rheum palmatum extract had a certain destructive effect on Foc TR4 mycelium, which produced a large number of branches and adhesions and caused uneven mycelial growth; among the four extraction fractions of the ethanolic crude extracts of Rheum palmatum, namely, petroleum ether, chloroform, ethyl acetate and n-butanol, the extract fraction of ethyl acetate showed the best inhibitory effects on the growth and spore germination of Foc TR4 mycelium, and the inhibition rate of mycelial growth could reach 85.68% when the concentration was 10 mg/mL. The EC50 value of ethyl acetate extract fraction of Rheum palmatum extract on Foc TR4 was 0.16 mg/mL.【Conclusion】The Rheum palmatum extract was screened out from 29 Chinses herbal medicine extracts with better inhibitory effect, and its main effective inhibitory component against banana Fusariu wilt Foc TR4 was in the ethyl acetate extract fraction was further clarified, which could provide a reference for the research and development of new pesticides from plant resources for the prevention and control of banana Fusarium wilt.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".