Additional file 1 of Biocatalytic cascade to polysaccharide amination
Bibliographic record
Abstract
Additional file 1: Figure S1. Acetophenone evaporation at 37 °C. Figure S2. The initial activity of CvATA and SpATA. Figure S3. Absorption spectra of red precipitates formed in the Q-NPEA assay. Figure S4. HPLC results confirming pyruvate depletion. Figure S5. Gel formation by acetyl bond formation in oxidized intermediates. Figure S6. Docking of galactomannan oligosaccharide on SpATA surface before and after alanine substitutions. Figure S7. PGBC in SpATA hosting the PLP cofactor. Figure S8. Discoloration of PLP catalyzed by CvATA. Figure S9. Formation of PMP-derived compounds in the discolored PLP solution. Figure S10. Initial rates of the wild-type SpATA and SpATA E407A measured on pyruvate with varying concentrations. Figure S11. The location of E407A mutation near the interfacial loops. Figure S12. Galactose oxidase inhibition by the aminated product measured using the ABTS assay. Table S1. A list of primers used in the site-directed mutagenesis of SpATA.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.013 |
| Meta-epidemiology (narrow) | 0.002 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.003 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.002 | 0.002 |
| Open science | 0.003 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.856 | 0.282 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".