Additional file 1 of Plasma derived extracellular vesicle biomarkers of microglia activation in an experimental stroke model
Bibliographic record
Abstract
Additional file 1: Table S1. Genbank accession number and primer sequences used for qPCR experiments. Figure S1. Nanoflow cytometry detection of TMEM119+/CD14+ EVs in comparison to standardized bead sizes. Figure S2. Transmission electron microscopy images of unlabeled EVs and CD14 or TMEM119 labelled EVs (via gold-conjugated secondary antibodies). Figure S3. A) Anterior to posterior boundaries of lesion following ET-1 injection delineated using thionin staining. B) Lesion areas demarcated within yellow ROI. Figure S4. qPCR of CD14 expression following LPS treatment of BV-2 microglia at either 100 ng/ml or 500 ng/ml for either 8 or 12 hours. Figure S5. Immunofluorescent staining of Iba1 and TMEM119 in BV-2 microglia and primary adult microglia with and without LPS exposure (24 hours, 500 ng/ml) Scale bar indicates 100 µm. Figure S6. Nanoflow cytometry measurement of TMEM119+/CD14+ and TMEM119+/MHC-II+ EVs in plasma samples from saline or endothelin-1 injected rats at baseline and 7 days post-surgery. Figure S7. Western Blot imaging files.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.012 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.003 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.825 | 0.135 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; the direct Gemma label and the distilled Codex classifier agree on what is shown here.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".