Additional file 2 of ERK activation via A1542/3 limonoids attenuates erythroleukemia through transcriptional stimulation of cholesterol biosynthesis genes
Bibliographic record
Abstract
Additional file 2: Figure 1: Structure of A1541-A1543 and cedrelone compounds and their biological activity in culture. Figure 2: Induction of the cholesterol biosynthesis genes by A1542. Q-RT-PCR analysis of expression of the indicated genes in HEL cells treated with DMSO or A1542 (1 μM) for 24 h. Figure 3: Induction of the cholesterol biosynthesis genes by A1543. Q-RT-PCR analysis of expression of the indicated genes in HEL cells treated with DMSO or A1543 (1 μM) for 24 h. Figure 4: Induction of the cholesterol biosynthesis genes by A1542 in SREBP1 knockdown cells. a-f Expression of the indicated genes in HEL cells treated with DMSO or A1542 (1 μM) for 24 h by Q-RT-PCR. Figure 5. Network map of genes interacts with EGR1 using STRING database. Figure 6: Suppression of the cholesterol biosynthesis genes induced by A1542 by AP1 inhibitor Tanshinone. Q-RT-PCR analysis of expression of the indicated genes in HEL cells treated with DMSO, A1542 (1 μM), Tanshinone (Tan IIA, 10 μM) and A1542 + Tan IIA for 24 h.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.012 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.003 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.002 | 0.002 |
| Open science | 0.002 | 0.001 |
| Research integrity | 0.002 | 0.001 |
| Insufficient payload (model declined to judge) | 0.871 | 0.183 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; the direct Gemma label and the distilled Codex classifier agree on what is shown here.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".