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Record W6920891851 · doi:10.6084/m9.figshare.26603794

Additional file 4 of Kinetic characterization of annotated glycolytic enzymes present in cellulose-fermenting Clostridium thermocellum suggests different metabolic roles

2024· article· en· W6920891851 on OpenAlexaff

Bibliographic record

VenueFigshare · 2024
Typearticle
Languageen
FieldMaterials Science
TopicEnzyme Structure and Function
Canadian institutionsUniversity of Manitoba
Fundersnot available
KeywordsClostridium thermocellumAldolase AThermophileThermusEnzymeThermotoga maritimaCrenarchaeota

Abstract

fetched live from OpenAlex

Additional file 4. Amino acid alignments of class II FBP aldolases. Figure S7. Including the characterized FBP aldolase of C. thermocellum currently under investigation; as well as the other annotated FBP aldolase encoded by C. thermocellum (C. thermocellum_hyp). Thermus aquaticus, Thermotoga maritima, Giardia lamblia, Helicobacter pylori, Geobacillus stearothermophilus all belong to class IIB, while E. coli, Corynebacterium glutamicum, Saccharomyces cerevisiae, Euglena gracilis belong to class IIA. The conserved residues implicated in binding the Zn2+ (D) and monovalent cation (M) have been identified based off stucturalr studies done the aldolase of E. coli [52]. Segments highlighted in black, identical residues, while letters that are bold represent similar residues. The sequences were aligned with MAFFT and the figure prepared with ESPript. The percent similarity between C. thermocellum characterized Aldoa and each of the other aldolases were placed at the end of the alignment.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Dataset · Consensus signal: none
Teacher disagreement score0.971
Threshold uncertainty score0.508

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.9710.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.213
Teacher spread0.202 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreDataset

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2024
Admission routes1
Has abstractyes

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